PXD051845

PXD051845 is an original dataset announced via ProteomeXchange.

Title	Genetic excision of the regulatory cardiac troponin I extension in high heart rate mammal clades
Description	Mammalian cardiac troponin I (cTnI) contains a highly conserved N-terminal extension harboring protein kinase A targets (Ser23/24) which are phosphorylated during ß-adrenergic stimulation to increase cardiomyocyte relaxation rate. Here, we show that the Ser23/24 encoding exon 3 of TNNI3 was pseudoexonized multiple times in shrews and moles to mimic Ser23/24 phosphorylation without adrenergic stimulation, thereby facilitating the evolution of exceptionally high resting heart rates (~1000 beats min-1). We further reveal alternative splicing of TNNI3 exon 3 in distantly related bat families with both cTnI isoforms being incorporated into cardiac myofibrils.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:50:56.021.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051845
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	mengdie zhang
SpeciesList	scientific name: Hipposideros armiger; NCBI TaxID: 186990; scientific name: Suncus murinus; NCBI TaxID: 9378;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-29 10:19:20	ID requested
1	2024-07-19 07:39:13	announced
⏵ 2	2024-10-22 06:50:56	announced	2024-10-22: Updated project metadata.

10.6019/PXD051845;

submitter keyword: Heart,LC-MS/MS,cTnI

Kai He
contact affiliation	Biochemistry Laboratory Building, Guangzhou University, No. 230, Waihuan West Road, Xiaoguwei Subdistrict, Panyu District, Guangzhou City, Guangdong Province.
contact email	hekai@gzhu.edu.cn
lab head
mengdie zhang
contact affiliation	Guangzhou University
contact email	zmd@e.gzhu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD051845

PRIDE project URI

• PRIDE
  1. PXD051845
     1. Label: PRIDE project
     2. Name: Genetic excision of the regulatory cardiac troponin I extension in high heart rate mammal clades