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PXD051841

PXD051841 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCyclin F - EXO1 axis controls cell cycle dependent execution of double strand break repair
DescriptionUbiquitination is a crucial post-translational modification required for the proper repair of DNA double-strand breaks (DSBs) induced by ionising radiation (IR). DSBs are mainly repaired through homologous recombination (HR) when template DNA is present and non-homologous end joining (NHEJ) in its absence. Additionally, microhomology-mediated end joining (MMEJ) and single strand annealing (SSA) provide back-up DSBs repair pathways. However, the mechanisms controlling their use remain poorly understood. By employing a high-resolution CRISPR screen of the ubiquitin system after IR, we systematically uncover genes required for cell survival and elucidate a critical role of the E3 ubiquitin ligase SCFcyclin F in cell cycle dependent DSB repair. We show that SCFcyclin F -mediated EXO1 degradation prevents DNA end resection in mitosis, allowing MMEJ to take place. Moreover, we identify a conserved cyclin F recognition motif, distinct from the one used by other cyclins, with broad implications in cyclin specificity for cell cycle control.
HostingRepositoryPRIDE
AnnounceDate2024-10-17
AnnouncementXMLSubmission_2024-10-17_05:15:18.457.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitteriolanda Vendrell
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-29 10:15:50ID requested
12024-10-17 05:15:19announced
Publication List
10.1126/sciadv.ado0636;
Yang H, Fouad S, Smith P, Bae EY, Ji Y, Lan X, Van Ess A, Buffa FM, Fischer R, Vendrell I, Kessler BM, D'Angiolella V, Cyclin F-EXO1 axis controls cell cycle-dependent execution of double-strand break repair. Sci Adv, 10(32):eado0636(2024) [pubmed]
Keyword List
submitter keyword: microhomology-mediated end joining, and cancer, ionising radiation, DNA damage repair,DNA damage response, DNA double strand breaks, EXO1, ubiquitination, single strand annealing, cell cycle, cyclin F
Contact List
Vincenzo D’Angiolella
contact affiliationRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Headington, Oxford OX3 9DS 2Edinburgh Cancer Research, CRUK Scotland Centre, Institute of Genetics and Cancer, University of Edinburgh, Crewe Road South, EH4 2XU, Edinburgh, UK
contact emailvdangio@ed.ac.uk
lab head
iolanda Vendrell
contact affiliationTarget Discovery Institute, NDMRB
contact emailiolanda.vendrell@ndm.ox.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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