PXD051839

PXD051839 is an original dataset announced via ProteomeXchange.

Title	Mass-spectrometry analysis of isolated brain vessels from vehicle treated Foxf2iECKO, vehicle-treated Ctrl, and AKB-9778 treated Foxf2iECKO mice
Description	To further characterize the role of endothelial Foxf2 in the maintenance of vascular function and examine a possible mediating effect of Tie2 signaling, we pharmacologically modulated Tie2 activity in mice and performed subsequent in vivo analyses together with studies on isolated brain vessels. Specifically, we applied AKB-9778, a selective small molecule inhibitor of vascular endothelial protein tyrosine phosphatase (Ptprb, VE-PTP) previously shown to stabilize the vasculature through Tie2 activation (Saharinen, et al., 2016; Shen et al., 2014). Mass-spectrometry analysis of isolated brain vessels from vehicle-treated Foxf2iECKO and Ctrl mice (Foxf2iECKO-Veh and Ctrl-Veh, respectively) confirmed the dysregulation of multiple proteins related to Tie2 signaling. Treatment with AKB-9778 for 48h restored their levels. Specifically, the abundance of Nos3, Nostrin and Gucy1b1 (implicated in nitric oxide metabolic process), Cldn5, Ctnnd1, Pecam1, and Rap1b (implicated in establishment of endothelial barrier), and Tie2, Flt1, and Itgb1 (implicated in angiogenesis) were all upregulated upon Tie2 activation. Collectively, these findings demonstrate that AKB-9778 rescues the deficiency of Tie2 signaling in mouse BECs lacking Foxf2.
HostingRepository	PRIDE
AnnounceDate	2025-09-02
AnnouncementXML	Submission_2025-09-02_09:40:05.137.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Mueller
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-29 03:15:40	ID requested
⏵ 1	2025-09-02 09:40:05	announced

Dataset with its publication pending

submitter keyword: small vessel disease,FOXF2, TIE2, microvessel

Stefan F.
contact affiliation	DZNE Munich, Neuroproteomics, Feodor-Lynen Str. 17, 81377 Munich, Germany
contact email	stefan.lichtenthaler@dzne.de
lab head
Stephan Mueller
contact affiliation	DZNE Munich Neuroproteomics
contact email	stephan.mueller@dzne.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051839
     1. Label: PRIDE project
     2. Name: Mass-spectrometry analysis of isolated brain vessels from vehicle treated Foxf2iECKO, vehicle-treated Ctrl, and AKB-9778 treated Foxf2iECKO mice