PXD051835

PXD051835 is an original dataset announced via ProteomeXchange.

Title	In vivo proteolytic profiling of the type I and type II metacaspases in Chlamydomonas reinhardtii exposed to salt stress
Description	Metacaspases are cysteine proteases present in plants, fungi and protists. While the association of metacaspases with cell death is studied in a range of organisms, their native substrates are largely unknown. Here, we explored the in vivo proteolytic landscape of the two metacaspases, CrMCA-I and CrMCA-II, present in the green freshwater alga Chlamydomonas reinhardtii, using N-terminal peptide labelling and enrichment during standard conditions and salt stress. Comparison between the cleavage events of CrMCA-I and CrMCA-II in metacaspase mutants revealed unique cleavage preferences and substrate specificity. Degradome analysis demonstrated relevance of the predicted metacaspase substrates to the physiology of Chlamydomonas reinhardtii cells and its adaptation during salt stress. Functional enrichment analysis indicated an involvement of CrMCA-I in photosynthesis and translation, while CrMCA-II seems to be important during the catabolism of carboxylic acids. Altogether, our findings suggest distinct cellular functions of the two metacaspases in C. reinhardtii during salt stress response.
HostingRepository	PRIDE
AnnounceDate	2024-06-11
AnnouncementXML	Submission_2024-06-11_04:52:56.826.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051835
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Evy Timmerman
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-29 01:23:42	ID requested
⏵ 1	2024-06-11 04:52:57	announced

10.6019/PXD051835;

submitter keyword: LC-MSMS,Chlamydomonas reinhardtii, metacaspase, N-terminal enrichment

Christiane Funk
contact affiliation	Department of Chemistry, Umeå University, Umeå, Sweden
contact email	christiane.funk@umu.se
lab head
Evy Timmerman
contact affiliation	Ghent University - VIB
contact email	evy.timmerman@vib-ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051835
     1. Label: PRIDE project
     2. Name: In vivo proteolytic profiling of the type I and type II metacaspases in Chlamydomonas reinhardtii exposed to salt stress