PXD051826

PXD051826 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	proteotoxic stress response in in-vitro 2 human neuronal models 3
Description	We used an untargeted pro-24 teomic approach using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify 25 and characterize time-series proteome-wide changes in dose-responsive mild and extreme proteo-26 toxic stress models in medulloblastoma [Daoy], neuroblastoma [SH-SY5Y] and differentiated SH-27 SY5Y neuronal cells. Combined global analysis of condition-time point datasets identified 1010 dif-28 ferentially expressed proteins (DEPs) in Daoy cells, 623 in SH-SY5Y cells, and 365 in differentiated 29 SH-SY5Y cells. The condition-specific analysis presents more DEPs and upregulated proteins in ex-30 treme heat stress with a relatively conservative and tight regulation in differentiated SH-SY5Y neu-31 ronal cells. Functional network analysis using ingenuity pathway analysis (IPA) identified path-32 ways associated with biological processes of protein, RNA, and amino acid metabolism, cellular 33 response to stress, and membrane trafficking as common to the three cell types.
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-24_16:23:50.044.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051826
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ayodele Alaiya
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-28 18:19:48	ID requested
⏵ 1	2025-08-24 16:23:50	announced

Publication List

Alaiya A, Alharbi BM, Shinwari Z, Rashid M, Albinhassan TH, Bouchama A, Alwesmi MB, Mohammad S, Malik SS, Proteomics Analysis of Proteotoxic Stress Response in In-Vitro Human Neuronal Models. Int J Mol Sci, 25(12):(2024) [pubmed]
10.3390/ijms25126787;
10.6019/PXD051826;

Alaiya A, Alharbi BM, Shinwari Z, Rashid M, Albinhassan TH, Bouchama A, Alwesmi MB, Mohammad S, Malik SS, Proteomics Analysis of Proteotoxic Stress Response in In-Vitro Human Neuronal Models. Int J Mol Sci, 25(12):(2024) [pubmed]

10.3390/ijms25126787;

10.6019/PXD051826;

Keyword List

submitter keyword: neurons
proteostasis
heat shock
proteotoxic stress
stress response
proteomics

submitter keyword: neurons

proteostasis

heat shock

proteotoxic stress

stress response

proteomics

Contact List

Dr_Ayodele Alaiya
contact affiliation	King Faisal Specialist Hospital and Research center Riyadh, Saudi Arabia
contact email	aalaiya@kfshrc.edu.sa
lab head
Ayodele Alaiya
contact affiliation	Therapeutics & Biomarker Discovery for Clinical Applications, Stem Cell Program, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia
contact email	aalaiya@kfshrc.edu.sa
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD051826
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD051826

PRIDE project URI

Repository Record List

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