PXD051808

PXD051808 is an original dataset announced via ProteomeXchange.

Title	Integrated Proteomics and Metabolomics Analysis of Sclerosis-Related Proteins and Femoral Head Necrosis Following Internal Fixation of Femoral Neck Fractures-1
Description	Femoral head necrosis (FHN) is a serious complication after femoral neck fractures (FNF), often linked to sclerosis around screw paths. Our study aimed to uncover the proteomic and metabolomic underpinnings of FHN and sclerosis using integrated proteomics and metabolomics analysis. We quantitatively identified differentially expressed proteins (DEPs) and metabolites (DEMs) among three groups: patients with FNF (Group A), sclerosis (Group B), and FHN (Group C). Through Kyoto Encyclopedia of Genes and Genomes and Gene Ontology enrichment analyses, we examined their roles. Our findings highlighted significant differences across the groups, with 218 DEPs and 44 DEMs identified between the sclerosis and FNF groups, 247 DEPs and 31 DEMs between the FHN and sclerosis groups, and a stark 682 DEPs and 94 DEMs between the FHN and FNF groups. Activities related to carbonate dehydratase and hydrolase were common between the FHN and sclerosis groups, whereas the extracellular region and lysosome were prevalent between the FHN and FNF groups. The study also emphasized the PI3K-Akt pathway’s involvement in sclerosis and FHN, alongside key metabolic pathways implicated in glycerophospholipid metabolism and retrograde endocannabinoid signaling. Western blotting confirmed the pivotal role of specific genes/proteins such as ITGB5, TNXB, CA II, and CA III in sclerosis and acid phosphatase 5 and cathepsin K in FHN. This comprehensive analysis elucidates the molecular mechanisms behind sclerosis and FHN and suggests potential biomarkers and therapeutic targets, paving the way for improved treatment strategies. Further validation of the findings is necessary to strengthen the robustness and reliability of the results.
HostingRepository	iProX
AnnounceDate	2024-04-27
AnnouncementXML	Submission_2024-04-27_20:12:39.742.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wangping Duan
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-27 20:11:48	ID requested
⏵ 1	2024-04-27 20:12:40	announced

Dataset with its publication pending

submitter keyword: Femoral head necrosis, Proteomics, Metabolomics, Sclerosis around screw paths

Pengcui Li
contact affiliation	Second Hospital of Shanxi Medical University
contact email	lpc1977@163.com
lab head
Wangping Duan
contact affiliation	Second Hospital of Shanxi Medical University
contact email	dwpsc2004121@163.com
dataset submitter

iProX dataset URI