PXD051800

PXD051800 is an original dataset announced via ProteomeXchange.

Title	Microfluidic chip-enabled proteome profiling of bacterial infection of macrophages reveals new pathogenic regulators of immune evasion.
Description	Microfluidic chip enabled cell sorting of non-phagocytosed K. pneumoniae cells from BALB/c macrophages during co-culture. These cell populations were analyzed to determine differences in phagocytosed K. pneumoniae versus non-phagocytosed at the protein level. This resulted in the identification of several iron-associated proteins associated with K. pneumoniae that evaded phagocytosis. Evidence in the literature indicated that secreted products from macrophages (i.e., cytokines) significantly increases expression of iron acquisition genes in bacteria, prompting our investigation (and the contents of our submission) into the changes in K. pneumoniae at the proteome-level when exposed to secretion products of macrophages exposed to pathogens (i.e., K. pneumoniae) versus unexposed, with specific emphasis on iron-associated proteins. It was confirmed that pathogen-exposed macrophage supernatant (i.e., activated macrophages induced higher production of iron-associated proteins in exposed K. pneumoniae compared to non-activated macrophages.
HostingRepository	PRIDE
AnnounceDate	2026-03-24
AnnouncementXML	Submission_2026-03-24_11:38:07.401.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chelsea Reitzel
SpeciesList	scientific name: Klebsiella pneumoniae; NCBI TaxID: NEWT:573;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-27 10:25:26	ID requested
⏵ 1	2026-03-24 11:38:08	announced

Dataset with its publication pending

submitter keyword: pneumoniae, 240, exploris, proteome,klebsiella, iron.

Jennifer Geddes-McAlister
contact affiliation	University of Guelph, Guelph, Ontario, Canada. Department of Molecular and Cellular Biology.
contact email	jgeddesm@uoguelph.ca
lab head
Chelsea Reitzel
contact affiliation	University of Guelph
contact email	reitzelc@uoguelph.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD051800

PRIDE project URI

• PRIDE
  1. PXD051800
     1. Label: PRIDE project
     2. Name: Microfluidic chip-enabled proteome profiling of bacterial infection of macrophages reveals new pathogenic regulators of immune evasion.