PXD051785

PXD051785 is an original dataset announced via ProteomeXchange.

Title	Rules of engagement for condensins and cohesins guide mitotic chromosome formation.
Description	During mitosis, interphase chromatin is rapidly converted into rod-shaped mitotic chromosomes. Using Hi-C, imaging, proteomics and polymer modeling, we determine how the activity and interplay between loop-extruding SMC motors accomplishes this dramatic transition. Our work reveals rules of engagement for SMC complexes that are critical for allowing cells to refold interphase chromatin into mitotic chromosomes. We find that condensin disassembles interphase chromatin loop organization by evicting or displacing extrusive cohesin. In contrast, condensin bypasses cohesive cohesins, thereby maintaining sister chromatid cohesion while separating the sisters. Studies of mitotic chromosomes formed by cohesin, condensin II and condensin I alone or in combination allow us to develop new models of mitotic chromosome conformation. In these models, loops are consecutive and not overlapping, implying that condensins do not freely pass one another but stall upon encountering each other. The dynamics of Hi-C interactions and chromosome morphology reveal that during prophase loops are extruded in vivo at ~1-3 kb/sec by condensins as they form a disordered discontinuous helical scaffold within individual chromatids.
HostingRepository	PRIDE
AnnounceDate	2025-12-16
AnnouncementXML	Submission_2025-12-16_13:45:19.905.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kumiko Samejima
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: NEWT:9031;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-26 12:32:03	ID requested
⏵ 1	2025-12-16 13:45:20	announced

10.1126/SCIENCE.ADQ1709;

submitter keyword: condensins, SLIAC, DIA,Mitosis, cohesins, chromosomes

Bill Earnshaw
contact affiliation	University of Edinburgh
contact email	bill.earnshaw@ed.ac.uk
lab head
Kumiko Samejima
contact affiliation	University of Edinburgh
contact email	kumiko.samejima@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD051785

PRIDE project URI

• PRIDE
  1. PXD051785
     1. Label: PRIDE project
     2. Name: Rules of engagement for condensins and cohesins guide mitotic chromosome formation.