PXD051783

PXD051783 is an original dataset announced via ProteomeXchange.

Title	RPM-1 in vivo affinity-purification proteomics using C. elegans
Description	To uncover putative RPM-1 protein-protein interactions, we performed a comprehensive series of in vivo affinity-purification proteomics experiments using C. elegans. We utilized integrated transgenes that express RPM-1 fused with a Protein G::Streptavidin binding peptide (GS) affinity tag expressed using the native rpm-1 promoter on an rpm-1 protein null background. Negative control animals expressed an integrated transgene where the GS tag was fused to GFP (GS::GFP) and expressed by the rpm-1 promoter. We also designed a biochemical “trap” using an RPM-1 ligase-dead (RPM-1 LD) site-directed mutant to identify ubiquitination substrates. RPM-1 LD would enrich substrates by trapping them in stalled ubiquitination complexes, as well as preventing proteasome-mediated degradation and elevating substrate levels. RPM-1-binding proteins that are not ubiquitination substrates would bind to both RPM-1 and RPM-1 LD.
HostingRepository	PRIDE
AnnounceDate	2024-11-19
AnnouncementXML	Submission_2024-11-19_15:16:39.092.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051783
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Brock Grill
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-26 12:03:58	ID requested
⏵ 1	2024-11-19 15:16:39	announced

10.6019/PXD051783;

submitter keyword: C. elegans, RPM-1

Brock Grill
contact affiliation	Unknown
contact email	brock.grill@seattlechildrens.org
lab head
Brock Grill
contact affiliation	University of Washington and Seattle Children's Research Institute
contact email	brock.grill@seattlechildrens.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051783
     1. Label: PRIDE project
     2. Name: RPM-1 in vivo affinity-purification proteomics using C. elegans