PXD051767

PXD051767 is an original dataset announced via ProteomeXchange.

Title	Acetylome analyses provide new insights into the effect of chronic intermittent hypoxia on hypothalamus-dependent endocrine metabolism impairment
Description	Background: Paediatric obstructive sleep apnoea (OSA) is a highly prevalent sleep disorder resulting in chronic intermittent hypoxia (CIH) that has been linked to metabolism and endocrine impairment. Protein acetylation, which is a frequently occurring posttranslational modification, plays pivotal roles in the regulation of hypothalamic processes. However, the effects of CIH-induced global protein acetylation on hypothalamic function and endocrine metabolism remain poorly understood. Methods: To bridge this knowledge gap, we conducted a study utilizing liquid chromatography–tandem mass spectrometry to analyse the lysine acetylome and proteome of the hypothalamus in healthy infantile mice exposed to 4 weeks of intermittent hypoxia (as a CIH model) compared to normoxic mice (as controls). Results: Our analysis identified and quantified 2699 lysine acetylation sites in 2453 proteins. These acetylated proteins exhibited disruptions primarily in endocrine metabolism, the citrate cycle (TCA cycle), synapse function, and circadian entrainment. Additionally, we observed significant downregulation of proteins that are known to be involved in endocrine hormone secretion. Metabolomic analysis of plasma suggested significant alterations in glycerophospholipid and amino acids metabolism, neuroactive ligand-receptor interaction and serotonergic synapse in children with OSA; these changes may represent potential mechanisms underlying the pathogenesis of OSA in children. Conclusion: This study aimed to elucidate the molecular mechanisms underlying CIH-induced alterations in protein acetylation within the hypothalamus. By providing valuable insights into the pathophysiological processes associated with CIH and their impacts on hypothalamic function, our findings contribute to a deeper understanding of the consequences stemming from CIH-induced changes in protein acetylation within the hypothalamus, as well as its potential role in endocrine impairment.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_05:18:03.197.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jun Tai
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-25 23:55:43	ID requested
⏵ 1	2024-10-17 05:18:05	announced

10.3390/biology13080559;

Kong Y, Ji J, Zhan X, Yan W, Liu F, Ye P, Wang S, Tai J, Acetylome Analyses Provide New Insights into the Effect of Chronic Intermittent Hypoxia on Hypothalamus-Dependent Endocrine Metabolism Impairment. Biology (Basel), 13(8):(2024) [pubmed]

submitter keyword: lysine acetylation, Paediatric obstructive sleep apnoea (OSA)., endocrine metabolism, chronic intermittent hypoxia,hypothalamus

Jun Tai
contact affiliation	Department of Otolaryngology, Head and Neck Surgery, Children’s Hospital Capital Institute of Paediatrics
contact email	trenttj@163.com
lab head
Jun Tai
contact affiliation	Department of Otolaryngology, Head and Neck Surgery, Children’s Hospital Capital Institute of Paediatrics
contact email	trenttj@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051767
     1. Label: PRIDE project
     2. Name: Acetylome analyses provide new insights into the effect of chronic intermittent hypoxia on hypothalamus-dependent endocrine metabolism impairment