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PXD051742

PXD051742 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMonolinks Matter: Rational Modification of a Cross-Linker for Improved Flexible Protein Structure Modeling
DescriptionChemical cross-linking/mass spectrometry (XL-MS) has emerged as a complementary tool for mapping interaction sites within protein networks as well as gaining moderate-resolution native structurale insight with minimal interference. XL-MS technology mostly relies on chemoselective reactions (crosslinking) between protein residues and a linker. Although complementary to crosslinks, monolinks are less commonly recognised as adjuvants for protein structure elucidation. Herein we report the rational design and synthesis of DSSO-carbamate, a novel cross-linker with increased selectivity for monolinks. Monolinks represent useful information for the elucidation of protein conformational changes and recent work has exploited their application in experimental crosslinking MS integrated protein structure prediction algorithms such as XL-MS-AlphaFold2 . its application supported by AlphaFold2 . Herein we report the rational design and synthesis of DSSO-carbamate, a novel cross-linker with increased selectivity for monolinks. Further, we demonstrate DSSO-carbamate’s capacity to facilitate improved protein structure prediction on account of its superior monolink generation.
HostingRepositoryPRIDE
AnnounceDate2025-05-06
AnnouncementXMLSubmission_2025-05-06_15:06:37.744.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD051742
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterKish Adoni
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ FT
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-25 09:45:25ID requested
12025-05-06 15:06:38announced
Publication List
10.1021/acs.analchem.4c05319;
10.6019/PXD051742;
Saridakis I, Adoni KR, Leischner T, Brutiu BR, Shaaban S, Ferrari G, Thalassinos K, Maulide N, Rational Modification of a Cross-Linker for Improved Flexible Protein Structure Modeling. Anal Chem, 97(2):1273-1280(2025) [pubmed]
Keyword List
submitter keyword: protein structure, Alphafold, crosslink, dsso-carbamate,Monolink
Contact List
Prof Konstantinos Thalassinos
contact affiliationUCL
contact emailk.thalassinos@ucl.ac.uk
lab head
Kish Adoni
contact affiliationUniversity College London
contact emailkish.95@live.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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