PXD051738

PXD051738 is an original dataset announced via ProteomeXchange.

Title	Binding of Akkermansia muciniphila to mucin is O-glycan specific
Description	The intestinal anaerobic bacterium Akkermansia muciniphila is specialized in the degradation of mucins, which are heavily O-glycosylated proteins that constitute the major components of the mucus lining the intestine. Despite that adhesion to mucins is considered critical for the persistence of A. muciniphila in the human intestinal tract, our knowledge of how this intestinal symbiont recognizes and binds to mucins is still limited. Here, we first show that the mucin-binding properties of A. muciniphila are independent of environmental oxygen concentrations and not abolished by pasteurization. We then dissected the mucin-binding properties of pasteurized A. muciniphila by use of a recently developed cell-based mucin array that enables display of the tandem repeats of human mucins with distinct O-glycan patterns and structures. We found that A. muciniphila recognizes the unsialylated LacNAc (Galβ1-4GlcNAc1-R) disaccharide selectively on core2 and core3 O-glycans. This disaccharide epitope is abundantly found on human colonic mucins capped by sialic acids, and we demonstrated that endogenous A. muciniphila neuraminidase activity can uncover the epitope and promote binding. In summary, our study provides insights into the mucin-binding properties important for colonization of a key mucin-foraging bacterium.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:43:16.492.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Noortje de Haan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-25 07:00:00	ID requested
1	2024-05-29 23:30:42	announced
⏵ 2	2024-10-22 06:43:16	announced	2024-10-22: Updated project metadata.

10.1038/S41467-024-48770-8;

submitter keyword: Akkermansia muciniphila,mucin, O-glycan

Noortje de Haan
contact affiliation	Center for Proteomics and Metabolomics, LUMC, The Netherlands
contact email	n.de_haan@lumc.nl
lab head
Noortje de Haan
contact affiliation	Copenhagen center for Glycomics, University of Copenhagen
contact email	ndehaan@sund.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD051738

PRIDE project URI

• PRIDE
  1. PXD051738
     1. Label: PRIDE project
     2. Name: Binding of Akkermansia muciniphila to mucin is O-glycan specific