PXD051737

PXD051737 is an original dataset announced via ProteomeXchange.

Title	Nanobodies as novel tools to monitor the mitochondrial fission factor Drp1
Description	In cells, mitochondria undergo constant fusion and fission. An essential factor for fission is the mammalian dynamin-related protein 1 (Drp1). Dysregulation of Drp1 has been linked to neurodegenerative diseases including Parkinson’s as well as cardiovascular diseases and cancer. Here, we developed nanobodies (Nbs) for proteomics, advanced microscopy and live cell imaging of Drp1. To specifically enrich endogenous Drp1 with interacting proteins for proteomics, we functionalized high-affinity Nbs as capture matrices. Considering the wide range of research applications, the presented Nb toolset will open up new possibilities for advanced functional studies of Drp1 in disease-relevant models.
HostingRepository	PRIDE
AnnounceDate	2024-05-29
AnnouncementXML	Submission_2024-05-29_15:35:42.160.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Claudia Cavarischia Rega
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-25 06:56:42	ID requested
⏵ 1	2024-05-29 15:35:42	announced

10.26508/LSA.202402608.;

submitter keyword: pulldown, proteomics, mitochondrial fission, Drp1,Nanobodies

Ulrich Rothbauer
contact affiliation	Pharmaceutical Biotechnology, Eberhard Karls University Tübingen, Germany
contact email	ulrich.rothbauer@uni-tuebingen.de
lab head
Claudia Cavarischia Rega
contact affiliation	Proteome Center Tuebingen Interfaculty Institute for Cell Biology University of Tuebingen
contact email	claudia.cavarischia-rega@uni-tuebingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD051737

PRIDE project URI

• PRIDE
  1. PXD051737
     1. Label: PRIDE project
     2. Name: Nanobodies as novel tools to monitor the mitochondrial fission factor Drp1