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PXD051730

PXD051730 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQualitative LC-MSMS analysis of Mus Musculus proteins
Description30 µg proteins per sample were reduced (20 mM DTT Sigma, room temperature RT, 1 h) and S-alkylated (50 mM IAA Sigma, 1 h, dark). The remaining IAA was quenched with 20 mM DTT for 1 h in the dark. The proteins were digested with 1:50(w/w enzyme:protein) MS-grade trypsin/lys-C mix (Thermo Scientific) for 24 h at RT. The enzyme digestion was quenched by lowering the pH with formic acid (Fisher) and the sample was desalted with ZipTip C18 (Merck-Millipore) column. Samples were resuspended in 10 µl of 0.2% formic acid prior LC-MS/MS analysis.
HostingRepositoryPRIDE
AnnounceDate2025-05-06
AnnouncementXMLSubmission_2025-05-06_15:04:37.649.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD051730
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterLiaqat Ali
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-25 00:07:01ID requested
12025-05-06 15:04:38announced
Publication List
10.1093/nar/gkaf280;
Hosny El Said N, Abdrabou W, Mahmood SR, Venit T, Idaghdour Y, Percipalle P, Nuclear actin-dependent Meg3 expression suppresses metabolic genes by affecting the chromatin architecture at sites of elevated H3K27 acetylation levels. Nucleic Acids Res, 53(7):(2025) [pubmed]
10.6019/PXD051730;
Keyword List
submitter keyword: Qualitative Proteomics, LC-MSMS,Mouse
Contact List
Piergiorgio Percipalle
contact affiliationNew York University Abu Dhabi, UAE
contact emailpp69@nyu.edu
lab head
Liaqat Ali
contact affiliationNew York University Abu Dhabi
contact emailla77@nyu.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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