PXD051730 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Qualitative LC-MSMS analysis of Mus Musculus proteins |
Description | 30 µg proteins per sample were reduced (20 mM DTT Sigma, room temperature RT, 1 h) and S-alkylated (50 mM IAA Sigma, 1 h, dark). The remaining IAA was quenched with 20 mM DTT for 1 h in the dark. The proteins were digested with 1:50(w/w enzyme:protein) MS-grade trypsin/lys-C mix (Thermo Scientific) for 24 h at RT. The enzyme digestion was quenched by lowering the pH with formic acid (Fisher) and the sample was desalted with ZipTip C18 (Merck-Millipore) column. Samples were resuspended in 10 µl of 0.2% formic acid prior LC-MS/MS analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-06 |
AnnouncementXML | Submission_2025-05-06_15:04:37.649.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD051730 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Liaqat Ali |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-04-25 00:07:01 | ID requested | |
⏵ 1 | 2025-05-06 15:04:38 | announced | |
Publication List
10.1093/nar/gkaf280; |
Hosny El Said N, Abdrabou W, Mahmood SR, Venit T, Idaghdour Y, Percipalle P, Nuclear actin-dependent Meg3 expression suppresses metabolic genes by affecting the chromatin architecture at sites of elevated H3K27 acetylation levels. Nucleic Acids Res, 53(7):(2025) [pubmed] |
10.6019/PXD051730; |
Keyword List
submitter keyword: Qualitative Proteomics, LC-MSMS,Mouse |
Contact List
Piergiorgio Percipalle |
contact affiliation | New York University Abu Dhabi, UAE |
contact email | pp69@nyu.edu |
lab head | |
Liaqat Ali |
contact affiliation | New York University Abu Dhabi |
contact email | la77@nyu.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD051730 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD051730
- Label: PRIDE project
- Name: Qualitative LC-MSMS analysis of Mus Musculus proteins