PXD051730

PXD051730 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Qualitative LC-MSMS analysis of Mus Musculus proteins
Description	30 µg proteins per sample were reduced (20 mM DTT Sigma, room temperature RT, 1 h) and S-alkylated (50 mM IAA Sigma, 1 h, dark). The remaining IAA was quenched with 20 mM DTT for 1 h in the dark. The proteins were digested with 1:50(w/w enzyme:protein) MS-grade trypsin/lys-C mix (Thermo Scientific) for 24 h at RT. The enzyme digestion was quenched by lowering the pH with formic acid (Fisher) and the sample was desalted with ZipTip C18 (Merck-Millipore) column. Samples were resuspended in 10 µl of 0.2% formic acid prior LC-MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_15:04:37.649.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051730
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Liaqat Ali
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-25 00:07:01	ID requested
⏵ 1	2025-05-06 15:04:38	announced

Publication List

10.1093/nar/gkaf280;
Hosny El Said N, Abdrabou W, Mahmood SR, Venit T, Idaghdour Y, Percipalle P, Nuclear actin-dependent Meg3 expression suppresses metabolic genes by affecting the chromatin architecture at sites of elevated H3K27 acetylation levels. Nucleic Acids Res, 53(7):(2025) [pubmed]
10.6019/PXD051730;

10.1093/nar/gkaf280;

Hosny El Said N, Abdrabou W, Mahmood SR, Venit T, Idaghdour Y, Percipalle P, Nuclear actin-dependent Meg3 expression suppresses metabolic genes by affecting the chromatin architecture at sites of elevated H3K27 acetylation levels. Nucleic Acids Res, 53(7):(2025) [pubmed]

10.6019/PXD051730;

Keyword List

submitter keyword: Qualitative Proteomics, LC-MSMS,Mouse

submitter keyword: Qualitative Proteomics, LC-MSMS,Mouse

Contact List

Piergiorgio Percipalle
contact affiliation	New York University Abu Dhabi, UAE
contact email	pp69@nyu.edu
lab head
Liaqat Ali
contact affiliation	New York University Abu Dhabi
contact email	la77@nyu.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD051730
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD051730

PRIDE project URI

Repository Record List

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