PXD051729
PXD051729 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of base excision repair protein complexes |
| Description | We recapitulated two main, cellular base excision repair (BER) complexes (herein termed Complex A, comprised of POLB and XRCC1 and Complex B, XRCC1-devoid complexes) by exploiting a separation-of-function mutant of POLB that does not bind to XRCC1, the V303-loop mutant POLB(TM). Cell lines were developed that expressed Flag-tagged-POLB(WT) and Flag-tagged-POLB(TM). To screen for binding partners of POLB or the POLB/XRCC1 complex, anti-Flag M2 affinity gel was used to immunoprecipitate proteins from cell lysates of LN428/Flag-POLB(WT), LN428/Flag-POLB(TM) or LN428/EGFP cells. The immunoprecipitates were loaded on SDS-PAGE gels and proteins were separated from low molecular weight reagents using short gel (~1cm) fractionation. The gels were stained by Coomassie blue. Gel regions were excised as indicated and processed for tryptic digestion. Liquid-chromatography Fourier transform mass spectrometry was used to measure the mass-to-charge (m/z) ratio, retention time, and intensity of more than 100,000 peptide signals that are detected in high-resolution full-scan mass spectra. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-04-26 |
| AnnouncementXML | Submission_2024-04-26_18:42:40.131.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xuemei Zeng |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | iodoacetamide - site C; L-methionine sulfoxide |
| Instrument | LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-04-24 20:18:16 | ID requested | |
| ⏵ 1 | 2024-04-26 18:42:40 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: TRIP12, POLB, Base Excision Repair, DNA Damage Response, Repair pathway choice |
Contact List
| Robert W. Sobol | |
|---|---|
| contact affiliation | Brown University |
| contact email | rwsobol@brown.edu |
| lab head | |
| Xuemei Zeng | |
| contact affiliation | University of Pittsburgh |
| contact email | xuzst1@yahoo.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000094615/ |
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