PXD051727

PXD051727 is an original dataset announced via ProteomeXchange.

Title	Proteomics of mature Bacillus subtilis spores in wild-type and mdfA deletion strains
Description	Endospore formation is a starvation-induced developmental pathway in Bacillus subtilis involving an asymmetrically-positioned cell division event that generates two cells, the smaller forespore and the larger mother cell. We previously demonstrated that sporulation is accompanied by metabolic differentiation of these two cells, and the depletion of enzymes in central metabolism from the developing spore. This disables spore metabolism and makes the forespore dependent on mother cell metabolism to produce metabolic building blocks for the biosynthesis of proteins, RNA and phospholipids. The mdfA (yjbA) gene was identified as being required for metabolic differentiation and this data set compares the proteome of wild-type and mdfA spores to assess the impact of metabolic differentiation on metabolism.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_15:04:48.661.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051727
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jelani Lyda
SpeciesList	scientific name: Bacillus subtilis PY79; NCBI TaxID: 1415167;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-24 19:35:37	ID requested
⏵ 1	2025-05-06 15:04:49	announced

10.6019/PXD051727;

10.1101/gad.352535.124;

Riley EP, Lyda JA, Reyes-Matte O, Sugie J, Kasu IR, Enustun E, Armbruster EG, Ravishankar S, Isaacson RL, Camp AH, Lopez-Garrido J, Pogliano K, sporulation. Genes Dev, 39(7-8):524-37(2025) [pubmed]

submitter keyword: acillus subtilis, sporulation, metabolic differentiation

Kit Pogliano
contact affiliation	University of California San Diego, School of Biological Science, United States
contact email	kpogliano@ucsd.edu
lab head
Jelani Lyda
contact affiliation	UCSD
contact email	jalyda@ucsd.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD051727

PRIDE project URI

• PRIDE
  1. PXD051727
     1. Label: PRIDE project
     2. Name: Proteomics of mature Bacillus subtilis spores in wild-type and mdfA deletion strains