PXD051725

PXD051725 is an original dataset announced via ProteomeXchange.

Title	Nascent proteome reveals key regulators in the development of alcoholic liver disease
Description	Alcoholic liver disease (ALD) poses a significant global health challenge, and the global proteome will change dynamically and dramatically for the response to alcohol stress. However, due to the lack of method for labeling and identification of tissue-specific nascent proteome in living mouse, the underlying molecular mechanism of the pathogenesis remains poorly understood. Here, we developed the stochastic orthogonal recoding of translation induced by AAV-delivered Cre (SORT-AC) strategy to precisely identify the nascent proteome in the hepatocyte of living mice. We applied this strategy to investigate the nascent proteome and underlying molecular mechanism in ALD mouse model. We identified 187 newly synthesized proteins involved in protein and metabolite processing in liver of ALD model mice. We found that upregulated Phb1/2 is responsible for the transcription of ethanol metabolism related genes and the downstream substrate, Hsp family proteins were responsible to protect from misfolded protein aggregation. Another downstream substrate of Phb1/2, Acsl1/5 participated in the lipid accumulation. Furthermore, the inhibition of Acsl1/5 protected cultured cells and mice from lipid accumulation by alcohol exposure, which might be a brand-new therapeutic target to antagonist the hepatocyte damage of ALD. More broadly, these results pave the new way for the nascent proteome investigation in living animal models in a tissue-specific manner.
HostingRepository	iProX
AnnounceDate	2025-05-20
AnnouncementXML	Submission_2025-05-26_20:00:38.465.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yulin Chen
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090; scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-24 18:32:34	ID requested
⏵ 1	2025-05-26 20:00:38	announced

Dataset with its publication pending

submitter keyword: SORT-AC, ALD, non-canonical amino acid

Shixian Lin
contact affiliation	Zhejiang University
contact email	sxlin@zju.edu.cn
lab head
Yulin Chen
contact affiliation	Zhejiang University
contact email	ychen209@qq.com
dataset submitter

iProX dataset URI