PXD051716

PXD051716 is an original dataset announced via ProteomeXchange.

Title	Short isoforms of E3 ligase RNF220 are broadly expressed and impact differentiation.
Description	The human RNF220 coding gene is located at Chr1: p34.1 (NCBI Gene ID: 55182). To date, four isoforms of the RNF220 protein have been described. Prior functional studies have investigated 70 kDa proteins, primarily isoform 1 but potentially isoform 3. Here, we show that the shorter isoforms of RNF220 that lack much of the N-terminus, the 353 AA isoform 2 and 308 AA isoform 4, are broadly expressed at high levels. While targets of RNF220 ubiquitination have been identified and their physiological roles elucidated [3,11], these studies have been limited to substrates of isoform 1. We hypothesized that the ubiquitous, highly expressed short isoforms may play a more fundamental role in most tissues. The shorter isoforms were found to perform distinct functions including in myoblast differentiation. These findings strongly argue for the investigation of these short isoforms in future studies on RNF220 function.
HostingRepository	PRIDE
AnnounceDate	2026-07-12
AnnouncementXML	Submission_2026-07-12_12:51:13.532.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Donald Wolfgeher
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-24 13:54:08	ID requested
⏵ 1	2026-07-12 12:51:14	announced

10.1016/j.mocell.2025.100250;

Choi S, Ha S, Wolfgeher DJ, Kim JW, Go YH, Cha HJ, Bae GU, Kron SJ, Kim WY, N-Terminal deleted isoforms of E3 ligase RNF220 are ubiquitously expressed and required for mouse muscle differentiation. Mol Cells, 48(9):100250(2025) [pubmed]

submitter keyword: muscle differentiation, Human, QE480, LFQ, variants, neurodegeneration, E3 ligase,RNF220

Dr. Woo-Young Kim
contact affiliation	College of Pharmacy, Sookmyung Women’s University, Seoul 04310, Republic of Korea.
contact email	wykim@sookmyung.ac.kr
lab head
Donald Wolfgeher
contact affiliation	University of Chicago
contact email	donw@uchicago.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD051716

PRIDE project URI

• PRIDE
  1. PXD051716
     1. Label: PRIDE project
     2. Name: Short isoforms of E3 ligase RNF220 are broadly expressed and impact differentiation.