PXD051711

PXD051711 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Galectin-3-binding protein inhibits extracellular heparan 6-O-endosulfatase Sulf-2
Description	Human extracellular 6-O-endosulfatases Sulf-1 and Sulf-2 are the only enzymes that post-synthetically alter the 6-O sulfation of heparan sulfate proteoglycans (HSPG), which regulates interactions of HSPG with many proteins. Oncogenicity of Sulf-2 in different cancers has been documented and we have shown that Sulf-2 is associated with poor survival outcomes in head and neck squamous cell carcinoma (HNSCC). In spite of its importance, limited information is available on direct protein-protein interactions of the Sulf-2 protein in the tumor microenvironment. In this study, we used monoclonal antibody (mAb) affinity purification and mass spectrometry to identify galectin-3-binding protein (LG3BP) as a highly specific binding partner of Sulf-2 in the conditioned media of HNSCC cell lines. We validated their direct interaction in vitro using recombinant proteins and have shown that the chondroitin sulfate (CS) covalently bound to the Sulf-2 influences the binding to LG3BP. We confirmed importance of the CS chain for the interaction by generating a mutant Sulf-2 protein that lacks the CS. Importantly, we have shown that the LG3BP inhibits Sulf-2 activity in vitro in a concentration dependent manner. As a consequence, the addition of LG3BP to a spheroid cell culture inhibited invasion of the HNSCC cells into Matrigel. Thus, Sulf-2 interaction with LG3BP may regulate physiological activity of the Sulf-2 enzyme as well as its activity in the tumor microenvironment.
HostingRepository	jPOST
AnnounceDate	2024-04-25
AnnouncementXML	Submission_2024-04-24_11:27:25.221.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aswini Panigrahi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-24 11:27:21	ID requested
⏵ 1	2024-04-24 11:27:26	announced

Publication List

Panigrahi A, Benicky J, Aljuhani R, Mukherjee P, Nov, á, kov, á Z, Ba, ř, inka C, Goldman R, -endosulfatse Sulf-2. bioRxiv, ():(2023) [pubmed]

Panigrahi A, Benicky J, Aljuhani R, Mukherjee P, Nov, á, kov, á Z, Ba, ř, inka C, Goldman R, -endosulfatse Sulf-2. bioRxiv, ():(2023) [pubmed]

Keyword List

submitter keyword: Sulf-2 mAb, LG3BP mAb, IP-MS

submitter keyword: Sulf-2 mAb, LG3BP mAb, IP-MS

Contact List

Radoslav Goldman
lab head
Aswini Panigrahi
contact affiliation	Georgetown University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003049/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003049/