PXD051709

PXD051709 is an original dataset announced via ProteomeXchange.

Title	Transit to Mars: low-dose γ-irradiation (Co60) induces small hormesis effect on Limnospira indica, a component of the MELiSSA life support system for space exploration
Description	In this study, two experiments using low-dose gamma irradiation (Co60, 70.4 µGy h-1 and 52.5 µGy h-1) to mimic the impact of 8-weeks of Mars transit flight of semi-continuous cultures of L. indica were performed. In the first experiment, the consecutive batches were 2 weeks long. After 2 weeks, 5% of the culture was transferred to a new flask and new medium was added. In the second experiment, the batches lasted 1 week. 25% of culture was transferred to the next run. In both approaches, the cultures were continuously illuminated (full PAR, 45 µmol photons m-2 s-1, LEDs) and shaken (120 rpm). A transient hormesis effect has been observed in the first 4 weeks of experiment 1, meaning that several growth and health parameters of the irradiated cultures increased compared to the controls in this time period. Two parameters could cause the pronounced effect of irradiation in exp. 1. Firstly, the higher variation in light and irradiation per cell ratio, caused by the different dilution schemes. Secondly, the higher irradiation dose due to the decay of the source between experiment 1 and 2.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_14:52:50.120.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051709
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Felice Mastroleo
SpeciesList	scientific name: Limnospira indica PCC 8005; NCBI TaxID: 376219;
ModificationList	No PTMs are included in the dataset
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-24 10:19:35	ID requested
⏵ 1	2025-05-06 14:52:50	announced

Fahrion J, Gupta S, Mastroleo F, Dussap CG, Leys N, . iScience, 28(3):111891(2025) [pubmed]

10.6019/PXD051709;

10.1016/j.isci.2025.111891;

submitter keyword: LC-MSMS, proteomics, irradiation,cyanobacteria, arthrospira

Natalie Leys
contact affiliation	Head Microbiology Unit, SCK CEN, Belgium
contact email	natalie.leys@sckcen.be
lab head
Felice Mastroleo
contact affiliation	SCK CEN
contact email	felice.mastroleo@sckcen.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD051709

PRIDE project URI

• PRIDE
  1. PXD051709
     1. Label: PRIDE project
     2. Name: Transit to Mars: low-dose γ-irradiation (Co60) induces small hormesis effect on Limnospira indica, a component of the MELiSSA life support system for space exploration