PXD051707

PXD051707 is an original dataset announced via ProteomeXchange.

Title	Dual functional POGases Encompassing O-glycanase and Adhesin Activities
Description	Mucin-type O-glycans on glycoproteins play many pivotal roles in biological processes, making O-glycosylation of biotherapeutics a potential critical quality attribute. Defining the structurefunction relationship of O-glycans is hindered by the lack of enzymatic tools to release all complex O-glycans from glycoproteins. Furthermore, glycans on host cells often serve as ligands for lectins/adhesins on bacteria for bacterium-host interactions, which are essential for the colonization of commensal bacteria and attachment/invasion of pathogens. More emerging adhesins require identification and characterization. To identify endo-α-Nacetylgalactosaminidases (O-glycanases, GH101) with broad substrate specificities, termed Peptide:O-Glycosidase (POGase), we screened putative candidates from 46 different bacteria with fluorogenic α2,3sialylCore 1 substrates, identified five POGase orthologs, and characterized one that releases sialylated complex O-glycans from glycopeptides, glycoproteins and biotherapeutics. Three unique peptide motifs differentiate the POGase existing in phylum Actinomycetota from known O-glycanases. Furthermore, while the POGases contain the GH101 domain, other domains at the C-terminus are required for efficient O-glycanase activity and possess binding activity to major glycans commonly decorating epithelial cells. The dual function POGases, which encompass O-glycanase and adhesin activities, will shed light on O-glycomics, quality assessment of O-glycoprotein drugs, microbiology, and pathogenesis of diseases that may lead to novel therapeutics.
HostingRepository	PRIDE
AnnounceDate	2025-02-25
AnnouncementXML	Submission_2025-02-25_08:06:57.043.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Bettinger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	complex glycosylation
Instrument	ultraflex; Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-24 09:41:54	ID requested
⏵ 1	2025-02-25 08:06:57	announced

10.1038/S41467-025-57143-8;

submitter keyword: enzyme, glycopeptides,O-glycans

Tongzhong Ju
contact affiliation	FDA
contact email	tongzhong.ju@fda.hhs.gov
lab head
John Bettinger
contact affiliation	FDA
contact email	john.bettinger@fda.hhs.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051707
     1. Label: PRIDE project
     2. Name: Dual functional POGases Encompassing O-glycanase and Adhesin Activities