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PXD051707

PXD051707 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDual functional POGases Encompassing O-glycanase and Adhesin Activities
DescriptionMucin-type O-glycans on glycoproteins play many pivotal roles in biological processes, making O-glycosylation of biotherapeutics a potential critical quality attribute. Defining the structurefunction relationship of O-glycans is hindered by the lack of enzymatic tools to release all complex O-glycans from glycoproteins. Furthermore, glycans on host cells often serve as ligands for lectins/adhesins on bacteria for bacterium-host interactions, which are essential for the colonization of commensal bacteria and attachment/invasion of pathogens. More emerging adhesins require identification and characterization. To identify endo-α-Nacetylgalactosaminidases (O-glycanases, GH101) with broad substrate specificities, termed Peptide:O-Glycosidase (POGase), we screened putative candidates from 46 different bacteria with fluorogenic α2,3sialylCore 1 substrates, identified five POGase orthologs, and characterized one that releases sialylated complex O-glycans from glycopeptides, glycoproteins and biotherapeutics. Three unique peptide motifs differentiate the POGase existing in phylum Actinomycetota from known O-glycanases. Furthermore, while the POGases contain the GH101 domain, other domains at the C-terminus are required for efficient O-glycanase activity and possess binding activity to major glycans commonly decorating epithelial cells. The dual function POGases, which encompass O-glycanase and adhesin activities, will shed light on O-glycomics, quality assessment of O-glycoprotein drugs, microbiology, and pathogenesis of diseases that may lead to novel therapeutics.
HostingRepositoryPRIDE
AnnounceDate2025-02-25
AnnouncementXMLSubmission_2025-02-25_08:06:57.043.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJohn Bettinger
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListcomplex glycosylation
Instrumentultraflex; Synapt MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-24 09:41:54ID requested
12025-02-25 08:06:57announced
Publication List
10.1038/S41467-025-57143-8;
Keyword List
submitter keyword: enzyme, glycopeptides,O-glycans
Contact List
Tongzhong Ju
contact affiliationFDA
contact emailtongzhong.ju@fda.hhs.gov
lab head
John Bettinger
contact affiliationFDA
contact emailjohn.bettinger@fda.hhs.gov
dataset submitter
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Dataset FTP location
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