PXD051699

PXD051699 is an original dataset announced via ProteomeXchange.

Title	Bioactive Peptide Profiling in Commercial Collagen Hydrolysates: Comparative Analysis Using Targeted LC-MS/MS Quantification
Description	The investigation of collagen hydrolysates (CHs) is essential due to their widespread use in health, cosmetic, and therapeutic industries, attributing to the presence of bioactive dipeptides (DPs) and tripeptides (TPs). This study developed a novel targeted LC-MS/MS method with propyl-chloroformate (PCF) derivatization to measure three bioactive peptides—Hydroxyprolyl-glycine (Hyp-Gly), Glycyl-prolyl-hydroxyproline (Gly-Pro-Hyp), and Prolyl-hydroxyproline (Pro-Hyp)—in CHs, with strong correlation coefficients (0.992, 1.000, and 0.995, respectively) and low limits of detection (LODs) of 1.40, 0.14, and 1.16 µM, respectively. Untargeted Data Dependent Acquisition (DDA) analyses measured peptide size distribution, while amino acid analysis assessed nutritional content. Analysis of ten commercial CHs revealed similar amino acid profiles but varied peptide lengths, indicating diverse hydrolysis conditions. Products with higher proportions of smaller peptides showed elevated levels of the targeted bioactive peptides, suggesting that smaller peptide size may increase bioactivity. These findings can inform the optimization of CH supplements, providing consumers with detailed peptide content for more informed choices.
HostingRepository	PRIDE
AnnounceDate	2024-06-09
AnnouncementXML	Submission_2024-06-09_03:33:08.416.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Merve Oztug
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-24 04:02:54	ID requested
⏵ 1	2024-06-09 03:33:08	announced

10.3390/MOLECULES29112592;

submitter keyword: Bioactive peptides, Propyl Chloroformate Derivatization,Collagen Hydrolysates, Peptide Profiling, LC-MS/MS

MERVE OZTUG
contact affiliation	TUBITAK UME, National Metrology Institute of TURKEY
contact email	merve.oztug@tubitak.gov.tr
lab head
Merve Oztug
contact affiliation	TUBITAK UME
contact email	merve.oztug@tubitak.gov.tr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD051699

PRIDE project URI

• PRIDE
  1. PXD051699
     1. Label: PRIDE project
     2. Name: Bioactive Peptide Profiling in Commercial Collagen Hydrolysates: Comparative Analysis Using Targeted LC-MS/MS Quantification