PXD051688

PXD051688 is an original dataset announced via ProteomeXchange.

Title	Synthetic Methylotrophic E. coli proteomics
Description	To effectively use methanol derived from CO2 or methane in E. coli, it is crucial to develop a fast-growing strain that can efficiently process methanol. We successfully developed a methylotrophic E. coli strain, SM6, which has a doubling time of 3.5 hours, surpassing the growth rates of typical native methylotrophs. This was achieved through evolutionary engineering that utilized dynamic copy number variation (CNV). The SM6 strain has demonstrated the ability to overcome the issue of formaldehyde-induced DNA-protein cross-linking (DPC) during its evolution, enabling rapid growth in methanol medium. Proteomics are conducted to understand the underlying metabolism / regulation change for the improved growth phenotype.
HostingRepository	PRIDE
AnnounceDate	2024-04-24
AnnouncementXML	Submission_2024-04-23_20:58:32.956.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051688
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	James C. Liao
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-23 20:45:40	ID requested
⏵ 1	2024-04-23 20:58:33	announced

10.6019/PXD051688;

submitter keyword: Synthetic methylotroph, methanol, Proteomics,E. coli, Synthetic Biology

James C. Liao
contact affiliation	Institute of Biological Chemistry, Academia SInica, Taiwan
contact email	liaoj@gate.sinica.edu.tw
lab head
James C. Liao
contact affiliation	Academia Sinica
contact email	liaoj@gate.sinica.edu.tw
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD051688

PRIDE project URI

• PRIDE
  1. PXD051688
     1. Label: PRIDE project
     2. Name: Synthetic Methylotrophic E. coli proteomics