PXD051679
PXD051679 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | LETMD1 regulates brown adipocyte thermogenesis through facilitation of mitochondrial protein import |
| Description | Thermogenic brown and beige adipocytes (BA) are powerful agents against the growing global obesity epidemic, as they break down lipids to generate heat. We and others have recently shown that LETM1-domain containing 1 (LETMD1) is a cold-induced protein essential for mitochondrial cristae structure and thermogenic function of BA. Still, how LETMD1 regulates thermogenesis of BA remains unclear. Here we first demonstrated that LETMD1 is a mitochondrial inner membrane protein. We next generated a BA-specific UCP1-Cre mediated Letmd1 knockout (Letmd1UKO) mouse model to show that LETMD1 plays a cell autonomous role in maintaining the structure and function of BA. We further uncovered robust mitochondrial autophagy (mitophagy) and protein aggregation within Letmd1UKO BA, in conjunction with elevated levels of reactive oxidative stress and mitochondrial hyperpolarization. We then used TurboID proximity labeling to identify candidate LETMD1-interacting proteins, many of which are associated with the mitochondrial protein import machinery and electron transport chain Complexes I and IV. We finally used quantitative proteomics to reveal an enrichment of Complex I and IV proteins within the insoluble protein aggregates. These results suggest that LETMD1 facilitates the mitochondrial import of respiratory chain proteins to maintain BA cristae structure and thermogenesis. |
| HostingRepository | jPOST |
| AnnounceDate | 2024-04-23 |
| AnnouncementXML | Submission_2024-04-23_19:03:42.600.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yi-Kai Liu |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide |
| Instrument | 6520A Quadrupole Time-of-Flight LC/MS; Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-04-23 19:00:00 | ID requested | |
| ⏵ 1 | 2024-04-23 19:03:43 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: LETMD1, brown adipocyte, mitochondria, protein import |
Contact List
| W. Andy Tao, Shihuan Kuang | |
|---|---|
| lab head | |
| Yi-Kai Liu | |
| contact affiliation | Purdue University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003047/ |
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