PXD051672

PXD051672 is an original dataset announced via ProteomeXchange.

Title	Impact of 5-FU on the proteome of HEK293T cells
Description	In this study, we wanted to examine the effects of 5-fluorouracil, a drug used in anticancer treatment, on the proteome of HEK293T cells (human cell culture). HEK293T cells were exposed to 5-fluorouracil (5-FU) using two different incubation strategies. In the first experiment, cells were incubated in 5-FU supplied medium and harvested after 24h acute exposure. The second experiment included a 24h exposure to 5-FU, followed by a recovery phase, were cells were incubated for another 6h in fresh medium without 5-FU. We performed shotgun proteomics of the respective tryptic digest of both experiments and compared the proteome of treated and untreated cells, respectively.
HostingRepository	PRIDE
AnnounceDate	2025-03-18
AnnouncementXML	Submission_2025-03-18_02:47:55.472.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051672
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Maximilian Berg
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-23 10:03:50	ID requested
⏵ 1	2025-03-18 02:47:56	announced

10.6019/PXD051672;

Berg M, Li C, Kaiser S, NAIL-MS reveals tRNA and rRNA hypomodification as a consequence of 5-fluorouracil treatment. Nucleic Acids Res, 53(4):(2025) [pubmed]

10.1093/nar/gkaf090;

submitter keyword: HEK293T, 5-fluorouracil, shotgun proteomics, human cell culture

Stefanie Kaiser
contact affiliation	Kaiser lab Goethe University Frankfurt Institute of Pharmaceutical Chemistry Max-von-Laue Str. 7-9 60438 Frankfurt am Main Germany
contact email	stefanie.kaiser@pharmchem.uni-frankfurt.de
lab head
Maximilian Berg
contact affiliation	Goethe University Frankfurt Institute of Pharmaceutical Chemistry Max-von-Laue Straße 7-9 60438 Frankfurt am Main
contact email	berg@pharmchem.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD051672

PRIDE project URI

• PRIDE
  1. PXD051672
     1. Label: PRIDE project
     2. Name: Impact of 5-FU on the proteome of HEK293T cells