PXD051672
PXD051672 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Impact of 5-FU on the proteome of HEK293T cells |
Description | In this study, we wanted to examine the effects of 5-fluorouracil, a drug used in anticancer treatment, on the proteome of HEK293T cells (human cell culture). HEK293T cells were exposed to 5-fluorouracil (5-FU) using two different incubation strategies. In the first experiment, cells were incubated in 5-FU supplied medium and harvested after 24h acute exposure. The second experiment included a 24h exposure to 5-FU, followed by a recovery phase, were cells were incubated for another 6h in fresh medium without 5-FU. We performed shotgun proteomics of the respective tryptic digest of both experiments and compared the proteome of treated and untreated cells, respectively. |
HostingRepository | PRIDE |
AnnounceDate | 2025-03-18 |
AnnouncementXML | Submission_2025-03-18_02:47:55.472.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD051672 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Maximilian Berg |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-04-23 10:03:50 | ID requested | |
⏵ 1 | 2025-03-18 02:47:56 | announced |
Publication List
10.6019/PXD051672; |
Berg M, Li C, Kaiser S, NAIL-MS reveals tRNA and rRNA hypomodification as a consequence of 5-fluorouracil treatment. Nucleic Acids Res, 53(4):(2025) [pubmed] |
10.1093/nar/gkaf090; |
Keyword List
submitter keyword: HEK293T, 5-fluorouracil, shotgun proteomics, human cell culture |
Contact List
Stefanie Kaiser | |
---|---|
contact affiliation | Kaiser lab Goethe University Frankfurt Institute of Pharmaceutical Chemistry Max-von-Laue Str. 7-9 60438 Frankfurt am Main Germany |
contact email | stefanie.kaiser@pharmchem.uni-frankfurt.de |
lab head | |
Maximilian Berg | |
contact affiliation | Goethe University Frankfurt Institute of Pharmaceutical Chemistry Max-von-Laue Straße 7-9 60438 Frankfurt am Main |
contact email | berg@pharmchem.uni-frankfurt.de |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD051672 |
PRIDE project URI |
Repository Record List
[ + ]