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PXD051672

PXD051672 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleImpact of 5-FU on the proteome of HEK293T cells
DescriptionIn this study, we wanted to examine the effects of 5-fluorouracil, a drug used in anticancer treatment, on the proteome of HEK293T cells (human cell culture). HEK293T cells were exposed to 5-fluorouracil (5-FU) using two different incubation strategies. In the first experiment, cells were incubated in 5-FU supplied medium and harvested after 24h acute exposure. The second experiment included a 24h exposure to 5-FU, followed by a recovery phase, were cells were incubated for another 6h in fresh medium without 5-FU. We performed shotgun proteomics of the respective tryptic digest of both experiments and compared the proteome of treated and untreated cells, respectively.
HostingRepositoryPRIDE
AnnounceDate2025-03-18
AnnouncementXMLSubmission_2025-03-18_02:47:55.472.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD051672
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMaximilian Berg
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-23 10:03:50ID requested
12025-03-18 02:47:56announced
Publication List
10.6019/PXD051672;
Berg M, Li C, Kaiser S, NAIL-MS reveals tRNA and rRNA hypomodification as a consequence of 5-fluorouracil treatment. Nucleic Acids Res, 53(4):(2025) [pubmed]
10.1093/nar/gkaf090;
Keyword List
submitter keyword: HEK293T, 5-fluorouracil, shotgun proteomics, human cell culture
Contact List
Stefanie Kaiser
contact affiliationKaiser lab Goethe University Frankfurt Institute of Pharmaceutical Chemistry Max-von-Laue Str. 7-9 60438 Frankfurt am Main Germany
contact emailstefanie.kaiser@pharmchem.uni-frankfurt.de
lab head
Maximilian Berg
contact affiliationGoethe University Frankfurt Institute of Pharmaceutical Chemistry Max-von-Laue Straße 7-9 60438 Frankfurt am Main
contact emailberg@pharmchem.uni-frankfurt.de
dataset submitter
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Dataset FTP location
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