PXD051646

PXD051646 is an original dataset announced via ProteomeXchange.

Title	Mutant RPL10 prevents large ribosomal subunit maturation
Description	The eukaryotic ribosome is a complex molecular machine responsible for the translation of mRNA to protein. Faulty ribosome production leads to decreased protein production, increased cellular stress, and often cell death. Previous research on ribosome biogenesis primarily focused on factors facilitating the proper assembly of the ribosome. By contrast, investigations into quality control mechanisms targeting abnormal pre-ribosomes remain relatively unexplored. We established a method to impede the maturation of a small subset of ribosomes in human cells. The assembly of the 60S large ribosomal subunit begins in the nucleolus, continues through the nucleoplasm, and concludes in the cytoplasm with the incorporation of the ribosomal protein uL16/RPL10 and the release of the late-acting biogenesis factors. We designed a system where incorporation of a mutant uL16 lacking residues 102-111 blocks the assembly of the maturing large subunit. To confirm that uL16mut incorporation blocks 60S maturation, we purified tagged uL16wt or uL16mut and identified associated proteins via western blotting and mass spectrometry analysis. We did not detect an interaction between uL16mut-containing large and subunits small subunit proteins, validating that the 60Smut subunits are trapped during biogenesis and unable to pair with small subunits to engage in active translation. We observed significant enrichment of late ribosome biogenesis factors, such as eIF6 and NMD3, upon uL16mut immunoprecipitation.
HostingRepository	PRIDE
AnnounceDate	2025-04-28
AnnouncementXML	Submission_2025-04-28_12:02:25.915.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shouling Xu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-22 15:03:48	ID requested
⏵ 1	2025-04-28 12:02:26	announced

10.1016/J.MOLCEL.2025.04.017;

submitter keyword: proteomics,Immunoprecipitation, ribosome biogenesis

SHOULING XU
contact affiliation	Carnegie MS Facility
contact email	sxu@ciw.edu
lab head
Shouling Xu
contact affiliation	Carnegie Institution at Stanford
contact email	sxu@ciw.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051646
     1. Label: PRIDE project
     2. Name: Mutant RPL10 prevents large ribosomal subunit maturation