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PXD051645

PXD051645 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleZNF574 is a Quality Control Factor Targeting Defective Ribosome Biogenesis Intermediates
DescriptionThe eukaryotic ribosome is a complex molecular machine responsible for the translation of mRNA to protein. Faulty ribosome production leads to decreased protein production, increased cellular stress, and often cell death. How defective ribosomes are detected and cleared is poorly understood. We modeled ribosome biogenesis failure in human cells and performed CRISPRi screens to identify quality control factors that cope with trapped biogenesis intermediates. We identified ZNF574 as the top hit. ZNF574 contains Cys2-His2 (C2H2) zinc finger binding domains that are known to interact with both DNA and RNA, providing a potential mechanism for recognizing and engaging defective ribosomes. ZNF574 localizes in the nucleoplasm and cellular speckles, similar to late ribosome biogenesis factors like eIF6 and NMD3. We confirmed that depletion of ZNF574 impedes the degradation of faulty ribosomes. We performed immunoprecipitation followed by mass spectrometric analysis. Our immunoprecipitation of GFP-tagged ZNF574 followed by mass spectrometry analysis of interacting proteins revealed strong enrichment for ribosomal proteins from the large subunit. Together, our data show that ZNF574 functions as a quality control factor that targets defective large subunits.
HostingRepositoryPRIDE
AnnounceDate2025-04-28
AnnouncementXMLSubmission_2025-04-28_12:02:13.909.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterShouling Xu
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-22 14:54:30ID requested
12025-04-28 12:02:14announced
Publication List
10.1016/J.MOLCEL.2025.04.017;
Keyword List
submitter keyword: proteomics, ribosome biogenesis,Immunoprecipitation mass spectrometry (IP-MS)
Contact List
SHOULING XU
contact affiliationCarnegie MS Facility
contact emailsxu@ciw.edu
lab head
Shouling Xu
contact affiliationCarnegie Institution at Stanford
contact emailsxu@ciw.edu
dataset submitter
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Dataset FTP location
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