PXD051602 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Global interactomics analysis of the human Duffy antigen receptor for chemokines |
Description | The Duffy antigen receptor for chemokines or DARC is a seven trans-membrane glycoprotein that is expressed primarily on the surface of red blood cells. Additionally, it is also expressed on the surface of post-venular endothelial cells, Purkinje cells of the cerebellum, inner ear hair cells, cells of the respiratory pathway and kidney epithelium. DARC binds to more than twenty different chemokines – belonging to both C-C and C-X-C subfamilies – making it the most promiscuous chemokine receptor. However, despite binding to such a large number of chemokines, no G-protein or β-arrestin signaling has been reported downstream to this receptor thus far. While canonical downstream signaling is not likely in erythrocytes considering their anucleate nature, DARC is also expressed in endothelial cells, and therefore, it is plausible that it signals through non-canonical signaling pathways. Considering that DARC does not couple to the canonical GPCR partners, we carried out a global interactome analysis upon stimulation of DARC-expressing HEK-293 cells with CCL7. We first performed a co-immunoprecipitation step under cross-linking condition to enrich DARC-interacting proteins followed by their detection using mass-spectrometry. In these experiments, we also used unstimulated cells as a reference, which were lysed and immunoprecipitated in parallel to subtract non-specific proteins. We carried out these experiments as four independent biological replicates. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-17 |
AnnouncementXML | Submission_2024-10-17_05:16:42.230.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | gagan jhingan |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 240 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-04-20 04:55:37 | ID requested | |
⏵ 1 | 2024-10-17 05:16:43 | announced | |
Publication List
10.1016/j.cell.2024.07.005; |
Saha S, Khanppnavar B, Maharana J, Kim H, Carino CMC, Daly C, Houston S, Sharma S, Zaidi N, Dalal A, Mishra S, Ganguly M, Tiwari D, Kumari P, Jhingan GD, Yadav PN, Plouffe B, Inoue A, Chung KY, Banerjee R, Korkhov VM, Shukla AK, Molecular mechanism of distinct chemokine engagement and functional divergence of the human Duffy antigen receptor. Cell, 187(17):4751-4769.e25(2024) [pubmed] |
Keyword List
submitter keyword: Duffy antigen receptor, G protein-coupled receptors, chemokine receptors, cellular signaling, cryogenic-electron microscopy |
Contact List
Arun Shukla |
contact affiliation | Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur 208016, India |
contact email | arshukla@iitk.ac.in |
lab head | |
gagan jhingan |
contact affiliation | VProteomics |
contact email | gaganjhingan@hotmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD051602 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD051602
- Label: PRIDE project
- Name: Global interactomics analysis of the human Duffy antigen receptor for chemokines