PXD051601
PXD051601 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomics of a mature female black widow spider (Latrodectus) from southeastern Pennsylvania |
| Description | In 2022 a vineyard in southeastern Pennsylvania was found to be infested with black widow spiders. Despite attempts to harvest proteins from the surface of an aluminum baseball bat, proteomic coverage was quite poor. In 2023, a mature female was temporarily stunned by the shockwave of an aluminum ballbat on concrete in a blow that altered the shape of both objects. This example of Latrodectus was transferred into a 15 mL falcon tube by a mass spectrometrist who made very undignified noises while making this transfer. The tube was rapidly sealed and the tube was placed at -20C before being transferred to -80C for storage. After the project was declined by multiple people in the laboratory a new student in the lab accepted this as his first proteomics project. The spider was carefully removed from the tube but the freeze/thaw process only allowed the separation of 2 distinct sections, titled "body" and "legs". These were homogenized in 4 fractions per section in a bead mill using 30 second pulses in 1x S-Trap lysis buffer. The resulting lysate was clarified by brief centrifugation and the lysates were digested using the S-Trap Mini spin columns following vendor protocols including the reduction and alkylation of cysteines with DTT and iodoacetamide. Peptides concentrations were determined by colorimetric assay (Pierce) and 400 nanograms of each were loaded onto EvoTips for analysis using a 30SPD method. A diaPASEF method on a TIMSTOF Flex instrument was used for analysis. Resulting samples were processed in SpectroNaut 18 using a combination of Latrodectus and Spider fastas from UniProt, as described in the manuscript. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-04-20 |
| AnnouncementXML | Submission_2024-04-20_04:59:40.527.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | ben |
| SpeciesList | scientific name: Latrodectus; common name: black widows; NCBI TaxID: 6923; |
| ModificationList | Carbamidomethyl; Oxidation |
| Instrument | timsTOF fleX |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-04-20 04:35:08 | ID requested | |
| ⏵ 1 | 2024-04-20 04:59:40 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Black widow spider, Latrodectus variolus, Latrodectus mactans |
Contact List
| Benjamin C Orsburn | |
|---|---|
| contact affiliation | Johns Hopkins |
| contact email | borsbur1@jhmi.edu |
| lab head | |
| ben | |
| contact affiliation | The Johns Hopkins School of Medicine |
| contact email | ben_orsburn3@hotmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000094573/ |
to receive all new ProteomeXchange dataset release announcements!
