PXD051595
PXD051595 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Evaluating single cell proteomics as a tool for understanding hepatotoxicity |
| Description | Bulk and single human primary hepatocytes were obtained from a commercial source from a single human donor. Cells were grown according to vendor protocols and treated with DMSO control or acetaminophen at the IC50 dose previously determined by R. Bruderer et al., 2015. Single cells were isolated using a Tecan UNO system using vendor protocols. 400 nanogram of bulk cell lysates from each condition were analyzed using diaPASEF using a TIMSTOF Flex system coupled to an EvoSep One system and using a 44 minute total gradient (30SPD). Single isolated cells were ran with a method with a compable run to run time and diaPASEF method using an EasyNLC 1200 system coupled to a TIMSTOF SCP system. In the case of the latter, cells were separated on a PepSep C-18 75 um column operating at 200 nL/min. Bulk cell lysates were used to create a library in SpectroNaut 18 from which was used for the analysis of all single cells. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-05-14 |
| AnnouncementXML | Submission_2025-05-14_05:47:08.975.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | ben orsburn |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Oxidation |
| Instrument | timsTOF SCP |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-04-19 17:50:45 | ID requested | |
| ⏵ 1 | 2025-05-14 05:47:09 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Hepatotoxicity, Single cell proteomics, diaPASEF |
Contact List
| Benjamin C Orsburn | |
|---|---|
| contact affiliation | Johns Hopkins |
| contact email | borsbur1@jhmi.edu |
| lab head | |
| ben orsburn | |
| contact affiliation | The University of Pittsburgh |
| contact email | ben_orsburn3@hotmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v07/MSV000094572/ |
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