PXD051563

PXD051563 is an original dataset announced via ProteomeXchange.

Title	Hybrid mass spectrometry applied across the production of antibody biotherapeutics.
Description	Post expression from the host cell, a bio-therapeutic will be subjected to several downstream processing steps prior to final formulation. Analysis of the product under various buffer conditions at each stage is desirable to show that the integrity of the sample is maintained and to monitor any potential product loss. In this study we examine how varying buffer conditions during downstream processing impact the structural integrity of a biopharmaceutical product, with a specific focus on the model IgG1 antibody, mAb4, provided by FUJIFILM Diosynth Biotechnologies. Flexibility, stability, aggregation propensity, and bulk properties were evaluated across the buffers of four sample points, including perfusion media, purification stages, and formulation buffer. Comparisons with Herceptin, an extensively studied IgG1 antibody, were conducted in a mass spectrometry-compatible buffer. Although mAb4 and Herceptin present with similar charge state distributions (CSD) in native mass spectrometry (MS) experiments, they exhibit distinct unfolding patterns during the activated ion mobility-mass spectrometry (aIM-MS) and differential scanning fluorimetry (DSF). The greater structural stability and aggregation onset temperature (Tagg) observed in Herceptin, are primarily attributed to the heavier glycosylation and kappa-class light chains present in Herceptin compared to the lambda-class light chains in mAb4. Hydrogen deuterium exchange mass spectrometry (HDX-MS) revealed that while mAb4 experiences substantial structural changes during purification, characterized by high flexibility, a low melting temperature (Tm), and prevalent repulsive protein-protein interactions, it eventually transitions to a highly compact and stable structure in buffers with higher salt concentrations and during formulation. Notably, in the formulated environment, the third constant domain (CH3) of the heavy chain retains flexibility and is identified as a region of interest for aggregation formation. Here we showcase the integration of MS and orthogonal techniques to attain comprehensive information which can be utilized early in the development stage to aid in decision-making regarding targeted mutations or to guide the design space of bioprocesses and formulation choices.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:49:27.684.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD051563
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Emilia Christofi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-18 09:18:42	ID requested
1	2024-07-12 03:01:30	announced
⏵ 2	2024-10-22 06:49:28	announced	2024-10-22: Updated project metadata.

10.6019/PXD051563;

ProteomeXchange project tag: Protein Misfolding and Aggregation (B/D-HPP), Hydrogen Deuterium Exchange (HDX-MS), Antibodies (B/D-HPP), Human Proteome Project

submitter keyword: dynamics, purification stages,monoclonal antibody, HDX-MS, formulation, perfusion media

Prof. Perdita Barran
contact affiliation	Michael Barber Centre for Collaborative Mass Spectrometry Manchester Institute of Biotechnology Department of Chemistry The University of Manchester
contact email	perdita.barran@manchester.ac.uk
lab head
Emilia Christofi
contact affiliation	The University of Manchester
contact email	emilia.christofi@manchester.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051563
     1. Label: PRIDE project
     2. Name: Hybrid mass spectrometry applied across the production of antibody biotherapeutics.