PXD051553

PXD051553 is an original dataset announced via ProteomeXchange.

Title	Size matters: Biomolecular compositions of small and large extracellular vesicles in the urine of glioblastoma patients
Description	Glioblastoma is a fatal and devastating primary brain tumour that poses significant challenges to accurate tumour monitoring and patient treatment. Substantial efforts are underway to develop accurate non-invasive liquid biopsy methods that can routinely assess glioblastoma markers in patient body fluids. We have recently shown that patient urine offers ready access to glioblastoma biomarkers within small extracellular vesicle populations (EVs; <200 nm membrane particles) and can accurately diagnose glioblastoma and reflect changes in glioblastoma pathophysiology. However, little is known about other urinary-EV populations in glioblastoma, and their capacity as biomarkers. In this study, we compared the biochemical properties of small (< 200 nm) and large (200-1000 nm) urinary-EVs using fourier-transform infrared spectroscopy and data independent acquisition mass spectrometry and identified significantly changing molecular distributions across the two EV populations in glioblastoma states. We also determined putative glioblastoma biomarkers associated with large urinary-EV biomarkers by ranking significant proteins with a logistic regression model. Our analysis revealed seven proteins capable of perfectly distinguishing all glioblastoma patients from healthy controls (AUC=1) and accurately classifying 96.7% of all cases as glioblastomas. Many significant large urinary-EV proteins also displayed consistent trends at different glioblastoma clinical timepoints, with protein abundance shifts post-surgery that return to pre-surgical levels at recurrence. Our findings demonstrate that both EV populations facilitate easy access to glioblastoma biomarkers for clinical diagnostics. Putative urinary-EV biomarkers described here merit further validation using comprehensive sets of longitudinal cohorts of glioblastoma urine.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_15:50:21.253.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susannah Hallal
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-18 03:46:24	ID requested
⏵ 1	2025-05-06 15:50:22	announced

Hallal SM, Sida LA, T, ű, zesi C Á, Shivalingam B, Sim HW, Buckland ME, Satgunaseelan L, Alexander KL, Size matters: Biomolecular compositions of small and large extracellular vesicles in the urine of glioblastoma patients. J Extracell Biol, 3(11):e70021(2024) [pubmed]

10.1002/jex2.70021;

submitter keyword: Gliioblastoma, Extracellular Vesicles, Liquid Biopsy, Urine

Kimberley Alexander
contact affiliation	Head of Brain Cancer Research, Chris O'Brien Lifehouse, Camperdown, Sydney, Australia
contact email	kim.alexander@lh.org.au
lab head
Susannah Hallal
contact affiliation	Chris O'Brien Lifehouse and University of Sydney
contact email	susannah.hallal@sydney.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051553
     1. Label: PRIDE project
     2. Name: Size matters: Biomolecular compositions of small and large extracellular vesicles in the urine of glioblastoma patients