PXD051551

PXD051551 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry identification of partners of the Toxoplasma gondii HCF101 protein
Description	Toxoplasma gondii is a parasitic protist that is the agent of toxoplasmosis. It is capable of infecting a wide variety of vertebrates, including humans. The infection is mainly asymptomatic in immunocompetent patients, but in case of immunosuppression or for the congenital form of toxoplasmosis it can lead to severe pathologies with a possible fatal outcome. Like for other eukaryotes, many key cellular functions in T. gondii involve proteins containing an iron-sulfur cluster as a cofactor. Cytosolic and nuclear iron-sulfur proteins depend on a specific pathway for assembling their iron-sulfur cofactor. We have investigated the T. gondii homolog of the HCF101 protein, initially characterized in plants as a chloroplast-based iron-sulfur transfer protein, by co-immunoprecipitating associated protein partners and identifying them by mass spectrometry. It confirmed that T. gondii HCF101 is not involved in plastid-based iron-sulfur metabolism, but in the biogenesis of cytosolic and nuclear iron-sulfur proteins instead.
HostingRepository	PRIDE
AnnounceDate	2025-01-24
AnnouncementXML	Submission_2025-01-24_02:25:19.538.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	valerie Rofidal
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: 5811;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-18 01:40:23	ID requested
⏵ 1	2025-01-24 02:25:19	announced

10.1371/JOURNAL.PBIO.3003028;

submitter keyword: Toxoplasma, iron-sulfur cluster, CIA pathway

Sébastien Besteiro
contact affiliation	LPHI, Univ Montpellier, CNRS, INSERM, Montpellier, France
contact email	sebastien.besteiro@inserm.fr
lab head
valerie Rofidal
contact affiliation	INRAE
contact email	valerie.rofidal@inrae.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051551
     1. Label: PRIDE project
     2. Name: Mass spectrometry identification of partners of the Toxoplasma gondii HCF101 protein