PXD051549

PXD051549 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis of a Toxoplasma gondii mutant of the HCF101 protein
Description	Toxoplasma gondii is a parasitic protist that is the agent of toxoplasmosis. It is capable of infecting a wide variety of vertebrates, including humans. The infection is mainly asymptomatic in immunocompetent patients, but in case of immunosuppression or for the congenital form of toxoplasmosis it can lead to severe pathologies with a possible fatal outcome. Like for other eukaryotes, many key cellular functions in T. gondii involve proteins containing an iron-sulfur cluster as a cofactor. Cytosolic and nuclear iron-sulfur proteins depend on a specific pathway for assembling their iron-sulfur cofactor. We have investigated the T. gondii homolog of the HCF101 protein, initially characterized in plants as a chloroplast-based iron-sulfur transfer protein, by generating a specific mutant on which we performed a quantitative proteomic analysis to get insights into its function in the parasites. We discovered that T. gondii HCF101 is not involved in plastid-based iron-sulfur metabolism, but in the biogenesis of cytosolic and nuclear iron-sulfur proteins instead. Control TATi ΔKu80 dataset is similar to the one provided in PRIDE entry PXD048386
HostingRepository	PRIDE
AnnounceDate	2025-01-24
AnnouncementXML	Submission_2025-01-24_03:04:45.215.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	valerie Rofidal
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: 5811;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-18 01:12:15	ID requested
⏵ 1	2025-01-24 03:04:45	announced

10.1371/JOURNAL.PBIO.3003028;

submitter keyword: Toxoplasma, iron-sulfur cluster, CIA pathway

Sébastien Besteiro
contact affiliation	LPHI, Univ Montpellier, CNRS, INSERM, Montpellier, France
contact email	sebastien.besteiro@inserm.fr
lab head
valerie Rofidal
contact affiliation	INRAE
contact email	valerie.rofidal@inrae.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD051549

PRIDE project URI

• PRIDE
  1. PXD051549
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis of a Toxoplasma gondii mutant of the HCF101 protein