PXD051549 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomic analysis of a Toxoplasma gondii mutant of the HCF101 protein |
Description | Toxoplasma gondii is a parasitic protist that is the agent of toxoplasmosis. It is capable of infecting a wide variety of vertebrates, including humans. The infection is mainly asymptomatic in immunocompetent patients, but in case of immunosuppression or for the congenital form of toxoplasmosis it can lead to severe pathologies with a possible fatal outcome. Like for other eukaryotes, many key cellular functions in T. gondii involve proteins containing an iron-sulfur cluster as a cofactor. Cytosolic and nuclear iron-sulfur proteins depend on a specific pathway for assembling their iron-sulfur cofactor. We have investigated the T. gondii homolog of the HCF101 protein, initially characterized in plants as a chloroplast-based iron-sulfur transfer protein, by generating a specific mutant on which we performed a quantitative proteomic analysis to get insights into its function in the parasites. We discovered that T. gondii HCF101 is not involved in plastid-based iron-sulfur metabolism, but in the biogenesis of cytosolic and nuclear iron-sulfur proteins instead. Control TATi ΔKu80 dataset is similar to the one provided in PRIDE entry PXD048386 |
HostingRepository | PRIDE |
AnnounceDate | 2025-01-24 |
AnnouncementXML | Submission_2025-01-24_03:04:45.215.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | valerie Rofidal |
SpeciesList | scientific name: Toxoplasma gondii; NCBI TaxID: 5811; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 240 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-04-18 01:12:15 | ID requested | |
⏵ 1 | 2025-01-24 03:04:45 | announced | |
Publication List
Keyword List
submitter keyword: Toxoplasma, iron-sulfur cluster, CIA pathway |
Contact List
Sébastien Besteiro |
contact affiliation | LPHI, Univ Montpellier, CNRS, INSERM, Montpellier, France |
contact email | sebastien.besteiro@inserm.fr |
lab head | |
valerie Rofidal |
contact affiliation | INRAE |
contact email | valerie.rofidal@inrae.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD051549 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD051549
- Label: PRIDE project
- Name: Quantitative proteomic analysis of a Toxoplasma gondii mutant of the HCF101 protein