PXD051528

PXD051528 is an original dataset announced via ProteomeXchange.

Title	Analysis of antibiotic response in Clinical Wound Pseudomonas aeruginosa isolates: Unveiling Proteome Dynamics of tobramycin tolerant phenotype
Description	Pseudomonas aeruginosa (PA) is an opportunistic human pathogen, causing serious chronic infections. PA can adapt efficiently to antibiotic stressors via different genotypic or phenotypic strategies such as resistance and tolerance. The adaptation regulatory system is not always very well understood. In this study, we use shotgun proteomics to investigate the system-level response to tobramycin in two clinical wound PA isolates and PAO1. We profiled each strain for its antibiotic drug-tolerant phenotype using supra-minimum inhibitory concentrations (supra-MIC) of tobramycin and apply proteomics to investigate the protein expression profiles. The MIC revealed that all isolates were susceptible to tobramycin but at supra-MIC concentrations at stationary growth, a degree of tolerance was observed for the isolates. We identified around 40 % of the total proteins encoded by the PA genome and highlighted shared and unique protein signatures for all isolates. Comparative proteome profiling in the absence of antibiotic treatment showed divergent fingerprints, despite similarities in the growth behavior of the isolates. In the presence of tobramycin, the isolates shared a common response in the downregulation of proteins involved in the two-component system, whereas stress response proteins were present at higher levels. Our findings provide insight into the use of proteomic tools to dissect the system-level response in clinical isolates in the absence and presence of antibiotic stress.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_08:01:51.243.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Biljana Mojsoska
SpeciesList	scientific name: Pseudomonas aeruginosa PAO1H2O; NCBI TaxID: 1147787; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-17 10:28:13	ID requested
⏵ 1	2024-10-17 08:01:51	announced

10.1016/J.MCPRO.2024.100861;

submitter keyword: shotgun proteomics,Pseudomons aeruginosa, tobramycin, antibiotic, tolerance

Mojsoska Biljana
contact affiliation	Principal Investigator -Associate Professor in Chemical Biology at the Department of Science and Environment-Roskilde University
contact email	biljana@ruc.dk
lab head
Biljana Mojsoska
contact affiliation	Roskilde University
contact email	biljana@ruc.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051528
     1. Label: PRIDE project
     2. Name: Analysis of antibiotic response in Clinical Wound Pseudomonas aeruginosa isolates: Unveiling Proteome Dynamics of tobramycin tolerant phenotype