PXD051522

PXD051522 is an original dataset announced via ProteomeXchange.

Title	Two-photon polymerization of miniaturised 3D scaffolds optimised for studies on glioblastoma multiforme in spaceflight-like microgravity conditions
Description	The obtainment of innovative models recapitulating the complex tumor architecture and activity in vitro is a challenging drive in the understanding of pathology molecular bases, yet a crucial passage to the identification of targets for advanced oncotherapy. Topography tuning and gravitational unloading of cell culture can represent useful means in tumor biomimicry process: in this work, both were chosen for modelling of glioblastoma multiforme, a highly aggressive neoplasm that affects the central nervous system and that has poorly effective pharmacological and radiological countermeasures. Tumor modeling was pursued by the application of two-photon polymerization to fast prototyping of scaffolds suitable for U87-MG glioma cell culture, and by the exposure of cell-scaffold hybrids to simulated microgravity (s-µg) by rotation on random positioning machine. Glioma cell response was investigated by microscopy, quantitative reverse transcription-polymerase chain reaction and proteomic analyses, denoting synergic regulatory effects of cell scaffolding and s-µg on markers of tumor cell growth, metabolism and invasiveness.
HostingRepository	PRIDE
AnnounceDate	2025-02-27
AnnouncementXML	Submission_2025-02-27_01:39:17.624.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martina Bartolucci
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-17 01:47:39	ID requested
⏵ 1	2025-02-27 01:39:18	announced

Dataset with its publication pending

submitter keyword: simulated microgravity, glioblastoma multiforme,tumor architecture, oncotherapy

Andrea Petreto
contact affiliation	IRCCS Istituto G Gaslini
contact email	a.petretto@gmail.com
lab head
Martina Bartolucci
contact affiliation	IRCCS Gaslini
contact email	smartibartolucci@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD051522

PRIDE project URI

• PRIDE
  1. PXD051522
     1. Label: PRIDE project
     2. Name: Two-photon polymerization of miniaturised 3D scaffolds optimised for studies on glioblastoma multiforme in spaceflight-like microgravity conditions