PXD051506

PXD051506 is an original dataset announced via ProteomeXchange.

Title	Cryptococcal infectome uncovers new therapeutic targets
Description	Mortality and morbidity rates of invasive fungal infections are rising as new pathogens emerge, antifungal resistance rates rise, and susceptible populations increase in numbers. Innovative strategies to identify and characterize novel druggable targets and investigate mechanisms of action driving fungal virulence and survival are urgently needed. For Cryptococcus neoformans, a human fungal pathogen with global distribution and elevated infectivity rates, especially among immunocompromised individuals, elucidation of mechanistic drivers of infection are limited. In this study, we developed an innovative pipeline to explore fluctuations in the fungal infectome combined with phenome fingerprinting to define three categories of putative fungal therapeutic candidates: antifungal, anti-virulence, and infection-relevant. Prioritization of an uncharacterized, conserved, and virulence-associated protein, CipC, explored distinct signatures of extracellular vesicles and pointed towards enhanced antigenic properties. A murine immunization trial revealed limited heightened protection with EVs from a deletion strain of cipC; however, an increased and diversified host immune response was observed, supporting a putative role in pan-fungal immunization. We report a comprehensive pipeline to define and explore novel drivers of fungal infectivity for the identification of new therapeutic targets to improve current treatment and protections strategies against fungal pathogens.
HostingRepository	PRIDE
AnnounceDate	2026-05-20
AnnouncementXML	Submission_2026-05-20_08:12:07.692.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jennifer Geddes-McAlister
SpeciesList	scientific name: Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii); NCBI TaxID: NEWT:235443; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-16 17:14:05	ID requested
⏵ 1	2026-05-20 08:12:08	announced

Dataset with its publication pending

submitter keyword: drug discovery, virulence,Quantitative proteomics, Immunization, fungal pathogenesis, infection, phenome

Jennifer Geddes-McAlister
contact affiliation	Molecular and Cellular Biology, University of Guelph, Canada
contact email	jgeddesm@uoguelph.ca
lab head
Jennifer Geddes-McAlister
contact affiliation	University of Guelph
contact email	jgeddesm@uoguelph.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051506
     1. Label: PRIDE project
     2. Name: Cryptococcal infectome uncovers new therapeutic targets