PXD051495

PXD051495 is an original dataset announced via ProteomeXchange.

Title	Cytokinetic Abscission in Toxoplasma gondii is governed by Protein Phosphatase 2A and the Daughter Cell Scaffold Complex
Description	Cytokinetic abscission is the last step of cell division during which the daughter cells physically separate through the generation of barriers in the form of new plasma membrane or cell wall. While the contractile ring is a prominent structure during cytokinesis in bacteria, fungi and animal cells, the mechanism is divergent in Apicomplexa. In Toxoplasma gondii, two daughter cells are formed within the intact mother cell by endodyogeny. The acquisition of plasma membrane at the end of the division cycle occurs by an unknown mechanism, when the mother cell disassembles, and the daughter cells emerge. Here we identified and functionally characterized the essential subunits of a predicted PP2A complex in T. gondii, named PP2A-2. The three subunits PP2A-A2, -B2 and -C2 exhibit a dynamic localization during parasite division. Their individual downregulation prevents the accumulation of plasma membrane at the division plane, resulting in a block of cytokinesis. Remarkably, the absence of cytokinetic abscission does not preclude division cycles and the consecutive progeny is able to successfully egress from the infected cells but fails to glide andinvade new host cells.
HostingRepository	PRIDE
AnnounceDate	2024-08-27
AnnouncementXML	Submission_2024-08-27_02:43:44.551.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gaelle Lentini
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: 5811; scientific name: Toxoplasma gondii; NCBI TaxID: NCBITaxon:5811;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-16 09:45:20	ID requested
1	2024-06-13 07:54:26	announced
⏵ 2	2024-08-27 02:43:45	announced	2024-08-27: Updated project metadata.

Marq JB, Gosetto M, Altenried A, Vadas O, Maco B, Dos Santos Pacheco N, Tosetti N, Soldati-Favre D, Lentini G, Cytokinetic abscission in Toxoplasma gondii is governed by protein phosphatase 2A and the daughter cell scaffold complex. EMBO J, 43(17):3752-3786(2024) [pubmed]

10.1038/s44318-024-00171-9;

submitter keyword: Toxoplasma gondii, cellular abscission, tachyzoites, PP2A complex, LC-ESI- MS/MS

Gaëlle Lentini
contact affiliation	Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland
contact email	gaelle.lentini@unige.ch
lab head
Gaelle Lentini
contact affiliation	Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland. Institute of Cell Biology, University of Bern, Bern, Switzerland
contact email	gaelle.lentini@unige.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD051495

PRIDE project URI

• PRIDE
  1. PXD051495
     1. Label: PRIDE project
     2. Name: Cytokinetic Abscission in Toxoplasma gondii is governed by Protein Phosphatase 2A and the Daughter Cell Scaffold Complex