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PXD051487

PXD051487 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLinking acetyl-CoA labelling and histone acetylation dynamics for accurate determination of acetylation rates via ordinary differential equations
DescriptionTo study complex biological systems, multi-omics methods have become an important tool. They integrate data from multiple biological layers, such as the metabolome and the proteome, to gain better insights of the processes happening. However, challenges remain both in sample preparation for measurements of different types of analytes as well as in the integration of different data types. Post-translational modifications such as acetylation of proteins are an example of a highly dynamic, reversible process that combines core metabolism with protein function. Histones are small proteins binding DNA that are highly acetylated in their N-terminal region. In this study, we utilise a simultaneous extraction method for acetyl-CoA and histones in combination with combined metabolic and chemical acetylation (CoMetChem) to track metabolic label incorporation into both acetyl-CoA and histones. Acetyl-CoA formation rates as well as histone acetylation- and deacetyation rates are modelled by ordinary differential equation, allowing the combination of data from acetyl-CoA and histone acetylation measurements into a unified model. We find that correcting for acetyl-CoA formation is necessary to accurately determine histone acetylation rates, particularly in systems with changes in upstream metabolism.
HostingRepositoryPRIDE
AnnounceDate2025-05-06
AnnouncementXMLSubmission_2025-05-06_14:40:42.591.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAnna-Sophia Egger
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-16 05:13:01ID requested
12025-05-06 14:40:43announced
Publication List
10.1016/j.molmet.2024.102032;
Keyword List
submitter keyword: Human, LC-MSMS, AcetylCoA, PTM, Histone, Acetylation, HEK93T
Contact List
Marcel Kwiatkowski
contact affiliationFunctional Proteo-Metabolomics Institute of Biochemistry University of Innsbruck
contact emailmarcel.kwiatkowski@uibk.ac.at
lab head
Anna-Sophia Egger
contact affiliationInstitut für Biochemie Universität Innsbruck
contact emailanna-sophia.egger@uibk.ac.at
dataset submitter
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Dataset FTP location
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