PXD051480

PXD051480 is an original dataset announced via ProteomeXchange.

Title	Molecular characterization of adhesives (glue lining pastes) used in restoration
Description	The molecular characterization of samples from works of art can provide valuable insights into the composition of ancient restoration materials and their conservation state. Here, we present a novel experimental protocol for the molecular characterization of a specific adhesive used in historical painting restoration, known as "glue lining pastes." Due to the high molecular complexity of these adhesives, we propose a multi-step extraction protocol to simultaneously recover and fractionate from a single microsample the three main classes of biomolecules contained in glue pastes (lipids, polysaccharides, and proteins). High-performance separation coupled with high-resolution MS techniques were applied to the isolated fractions to identify specific components. The proposed method was optimized using test specimens of various traditional glue pastes applied to canvases and successfully applied to a historical glue paste sample from the 17th-century painting "La fuga in Egitto," part of the Pagliara collection at the University Suor Orsola Benincasa (Naples, Italy). The data collected in this work provide insights into the specific recipe used for adhesive preparation, supporting artistic and historical interpretations, and contributing to a broader understanding of old restoration practices.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_05:05:36.480.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brunella Cipolletta
SpeciesList	scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: 9986; scientific name: Acipenser sturio; NCBI TaxID: 61674; scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565; scientific name: Secale cereale; NCBI TaxID: 4550; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: linum usitatissimum; NCBI TaxID: 4006;
ModificationList	2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-16 02:13:28	ID requested
⏵ 1	2024-10-17 05:05:37	announced

10.1021/acs.analchem.4c02138;

Cipolletta B, Morelli M, Perlingieri C, Somma PI, Amoresano A, Marino G, Carpentieri A, Molecular Characterization of Adhesives (Glue Lining Pastes) Used in Restoration. Anal Chem, 96(42):16551-16560(2024) [pubmed]

submitter keyword: Restoration, Extraction, GC-MS, Proteomics,Mass spectrometry, Glue lining pastes, LC-MS/MS

Andrea Carpentieri
contact affiliation	Department of Chemical Sciences, University of Naples Federico II
contact email	andrea.carpentieri@unina.it
lab head
Brunella Cipolletta
contact affiliation	Department of Chemical Sciences, University of Naples Federico II
contact email	brunella.cipolletta@unina.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051480
     1. Label: PRIDE project
     2. Name: Molecular characterization of adhesives (glue lining pastes) used in restoration