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PXD051457

PXD051457 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBiotinylated MMH2 pulldown LCMS
DescriptionHere, we present structural and mechanistic studies that define a trans-labeling covalent molecular glue mechanism. We found that a novel series of BRD4 molecular glue degraders act by recruiting the CUL4-DCAF16 ligase to the second bromodomain of BRD4. To gain a deeper understanding into the potential off-target effects of more reactive degraders, we conducted streptavidin pulldowns of K562 lysate incubated with engineered MMH2-Biotin, a probe molecule that appends linker-biotin onto the second exit vector of MMH2.
HostingRepositoryPRIDE
AnnounceDate2025-05-06
AnnouncementXMLSubmission_2025-05-06_14:02:58.700.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAnna Schmoker
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-15 12:06:43ID requested
12025-05-06 14:02:59announced
Publication List
10.1038/s41589-024-01668-4;
Li YD, Ma MW, Hassan MM, Hunkeler M, Teng M, Puvar K, Rutter JC, Lumpkin RJ, Sandoval B, Jin CY, Schmoker AM, Ficarro SB, Cheong H, Metivier RJ, Wang MY, Xu S, Byun WS, Groendyke BJ, You I, Sigua LH, Tavares I, Zou C, Tsai JM, Park PMC, Yoon H, Majewski FC, Sperling HT, Marto JA, Qi J, Nowak RP, Donovan KA, S, ł, abicki M, Gray NS, Fischer ES, Ebert BL, Template-assisted covalent modification underlies activity of covalent molecular glues. Nat Chem Biol, 20(12):1640-1649(2024) [pubmed]
Keyword List
submitter keyword: Human, LC-MSMS, molecular glue
Contact List
Eric Fischer
contact affiliationBiological Chemistry and Molecular Pharmacology, Harvard Medical School Cancer Biology, Dana-Farber Cancer Institute
contact emailefischer@crystal.harvard.edu
lab head
Anna Schmoker
contact affiliationDartmouth Cancer Center, Dartmouth Colleg
contact emailaschmoker@crystal.harvard.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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