PXD051457

PXD051457 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Biotinylated MMH2 pulldown LCMS
Description	Here, we present structural and mechanistic studies that define a trans-labeling covalent molecular glue mechanism. We found that a novel series of BRD4 molecular glue degraders act by recruiting the CUL4-DCAF16 ligase to the second bromodomain of BRD4. To gain a deeper understanding into the potential off-target effects of more reactive degraders, we conducted streptavidin pulldowns of K562 lysate incubated with engineered MMH2-Biotin, a probe molecule that appends linker-biotin onto the second exit vector of MMH2.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_14:02:58.700.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anna Schmoker
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-15 12:06:43	ID requested
⏵ 1	2025-05-06 14:02:59	announced

Publication List

10.1038/s41589-024-01668-4;
Li YD, Ma MW, Hassan MM, Hunkeler M, Teng M, Puvar K, Rutter JC, Lumpkin RJ, Sandoval B, Jin CY, Schmoker AM, Ficarro SB, Cheong H, Metivier RJ, Wang MY, Xu S, Byun WS, Groendyke BJ, You I, Sigua LH, Tavares I, Zou C, Tsai JM, Park PMC, Yoon H, Majewski FC, Sperling HT, Marto JA, Qi J, Nowak RP, Donovan KA, S, ł, abicki M, Gray NS, Fischer ES, Ebert BL, Template-assisted covalent modification underlies activity of covalent molecular glues. Nat Chem Biol, 20(12):1640-1649(2024) [pubmed]

10.1038/s41589-024-01668-4;

Li YD, Ma MW, Hassan MM, Hunkeler M, Teng M, Puvar K, Rutter JC, Lumpkin RJ, Sandoval B, Jin CY, Schmoker AM, Ficarro SB, Cheong H, Metivier RJ, Wang MY, Xu S, Byun WS, Groendyke BJ, You I, Sigua LH, Tavares I, Zou C, Tsai JM, Park PMC, Yoon H, Majewski FC, Sperling HT, Marto JA, Qi J, Nowak RP, Donovan KA, S, ł, abicki M, Gray NS, Fischer ES, Ebert BL, Template-assisted covalent modification underlies activity of covalent molecular glues. Nat Chem Biol, 20(12):1640-1649(2024) [pubmed]

Keyword List

submitter keyword: Human, LC-MSMS, molecular glue

submitter keyword: Human, LC-MSMS, molecular glue

Contact List

Eric Fischer
contact affiliation	Biological Chemistry and Molecular Pharmacology, Harvard Medical School Cancer Biology, Dana-Farber Cancer Institute
contact email	efischer@crystal.harvard.edu
lab head
Anna Schmoker
contact affiliation	Dartmouth Cancer Center, Dartmouth Colleg
contact email	aschmoker@crystal.harvard.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD051457
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD051457

PRIDE project URI

Repository Record List

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