PXD051457
PXD051457 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Biotinylated MMH2 pulldown LCMS |
Description | Here, we present structural and mechanistic studies that define a trans-labeling covalent molecular glue mechanism. We found that a novel series of BRD4 molecular glue degraders act by recruiting the CUL4-DCAF16 ligase to the second bromodomain of BRD4. To gain a deeper understanding into the potential off-target effects of more reactive degraders, we conducted streptavidin pulldowns of K562 lysate incubated with engineered MMH2-Biotin, a probe molecule that appends linker-biotin onto the second exit vector of MMH2. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-06 |
AnnouncementXML | Submission_2025-05-06_14:02:58.700.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Anna Schmoker |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-04-15 12:06:43 | ID requested | |
⏵ 1 | 2025-05-06 14:02:59 | announced |
Publication List
10.1038/s41589-024-01668-4; |
Li YD, Ma MW, Hassan MM, Hunkeler M, Teng M, Puvar K, Rutter JC, Lumpkin RJ, Sandoval B, Jin CY, Schmoker AM, Ficarro SB, Cheong H, Metivier RJ, Wang MY, Xu S, Byun WS, Groendyke BJ, You I, Sigua LH, Tavares I, Zou C, Tsai JM, Park PMC, Yoon H, Majewski FC, Sperling HT, Marto JA, Qi J, Nowak RP, Donovan KA, S, ł, abicki M, Gray NS, Fischer ES, Ebert BL, Template-assisted covalent modification underlies activity of covalent molecular glues. Nat Chem Biol, 20(12):1640-1649(2024) [pubmed] |
Keyword List
submitter keyword: Human, LC-MSMS, molecular glue |
Contact List
Eric Fischer | |
---|---|
contact affiliation | Biological Chemistry and Molecular Pharmacology, Harvard Medical School Cancer Biology, Dana-Farber Cancer Institute |
contact email | efischer@crystal.harvard.edu |
lab head | |
Anna Schmoker | |
contact affiliation | Dartmouth Cancer Center, Dartmouth Colleg |
contact email | aschmoker@crystal.harvard.edu |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD051457 |
PRIDE project URI |
Repository Record List
[ + ]