PXD051453

PXD051453 is an original dataset announced via ProteomeXchange.

Title	Salivary Proteomics and Metaproteomics Identifies Distinct Molecular and Taxonomic Signatures Of Type 2 Diabetes
Description	Background: Saliva is an attractive body fluid for non-invasive biomarker discovery, containing both human and microbial components, associated with various chronic diseases. Type-2 diabetes (T2D) imposes a significant health and socio-economic burden. Prior research on T2D salivary microbiome utilised methods such as metagenomics, metatranscriptomics, 16S rRNA sequencing, and low-throughput proteomics. Results: We conducted ultra-fast, in-depth MS-based profiling of saliva from 15 T2D individuals and 15 age/BMI-matched healthy controls (HC). Using state-of-the-art proteomics, over 4500 proteins were identified in 21 minutes of analysis, including both human and bacterial proteins. Analysis revealed signatures of altered immune, lipid- and glucose-metabolism regulatory systems, increased oxidative stress, and possible precancerous changes in T2D saliva. Bacterial genera Neisseria and Corynebacterium showed biomarker potential, offering insights into disease pathophysiology and microbial applications for T2D management. Conclusions: This study provides a comprehensive map of salivary proteins and microbial communities, serving as a resource for better understanding of T2D pathophysiology. The identified biomarkers offer potential for improved diagnostics and therapeutic strategies in T2D and associated long-term complications.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_14:29:53.643.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Diana Samodova-Sommer
SpeciesList	scientific name: Saccharomyces cerevisiae; NCBI TaxID: NCBITaxon:4932; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-15 09:38:42	ID requested
⏵ 1	2025-05-06 14:29:54	announced

Samodova D, Stankevic E, S, ø, ndergaard MS, Hu N, Ahluwalia TS, Witte DR, Belstr, ø, m D, Lubberding AF, Jagtap PD, Hansen T, Deshmukh AS, Salivary proteomics and metaproteomics identifies distinct molecular and taxonomic signatures of type-2 diabetes. Microbiome, 13(1):5(2025) [pubmed]

10.1186/s40168-024-01997-5;

submitter keyword: Protein, Peptide,Metaproteomics, DIA-PASEF, Microbiome, Saliva, Type-2-diabetes, Biomarker, Genus

Atul Shahaji Deshmukh
contact affiliation	Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark
contact email	atul.deshmukh@sund.ku.dk
lab head
Diana Samodova-Sommer
contact affiliation	Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen
contact email	diana.samodova@sund.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD051453

PRIDE project URI

• PRIDE
  1. PXD051453
     1. Label: PRIDE project
     2. Name: Salivary Proteomics and Metaproteomics Identifies Distinct Molecular and Taxonomic Signatures Of Type 2 Diabetes