PXD051447

PXD051447 is an original dataset announced via ProteomeXchange.

Title	The extracellular matrix of meat: a characterization towards a 3D scaffold for cultured meat
Description	The use of an animal component-free scaffold to culture muscle cells on is a potential approach for producing (hybrid) cultured meat. However, to develop a plant-based scaffold with optimal mechanical characteristics for muscle cell growth, differentiation and maturation, it is essential to characterize the natural extracellular matrix (ECM) found in conventional meat. Therefore, this research initially involved isolating the ECM by removing muscle cells through 0.5 % SDS decellularization. Subsequently, various structural parameters of both fresh meat (representing the upper limit) and decellularized meat (representing the lower limit) were measured. Effective removal of cells and adequate preservation of the ECM's main structure and components were confirmed through Picogreen assay, proteomic analysis, and determination of glycosaminoglycan (GAG) content. Samples underwent amplitude sweeps, texture analyses and internal structure visualization. The conditions resembling the natural ECM of muscle cells lie between the determined upper and lower limits for each parameter.
HostingRepository	PRIDE
AnnounceDate	2025-07-17
AnnouncementXML	Submission_2025-07-17_02:00:26.940.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	BRAY FABRICE
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-15 06:19:13	ID requested
⏵ 1	2025-07-17 02:00:27	announced

10.1016/J.FOOSTR.2025.100447;

submitter keyword: proteomics, detergent,Cultured meat, decellularization, extracellular matrix

fabrice bray
contact affiliation	Laboratoire MSAP UAR 3290 CNRS Bâtiment C4 Avenue Paul Langevin Cité Scientifique 59655 Villeneuve D'ascq 03 20 33 71 12 https://msap-lab.fr/
contact email	fabrice.bray@univ-lille.fr
lab head
BRAY FABRICE
contact affiliation	MSAP USR 3290
contact email	fabrice.bray@univ-lille.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD051447

PRIDE project URI

• PRIDE
  1. PXD051447
     1. Label: PRIDE project
     2. Name: The extracellular matrix of meat: a characterization towards a 3D scaffold for cultured meat