PXD051440

PXD051440 is an original dataset announced via ProteomeXchange.

Title	Two distinct Trypanosoma eIF4F complexes co-exist, bind different mRNAs and are regulated during nutritional stress
Description	Many eIF4F subunits and PABP paralogues are found in trypanosomes: six eIF4E, five eIF4G, one eIF4A and two PABPs. They are expressed simultaneously and assemble into different complexes, contrasting the situation in metazoans that use distinct complexes in different cell types or developmental stages. Each eIF4F complex has its own proteins, mRNAs and, consequently, a distinct function. We set out to study the function and regulation of the two major eIF4F complexes of Trypanosoma cruzi. We identified the associated proteins and mRNAs of eIF4E3 and eIF4E4 in cells in exponential growth and in nutritional stress. Upon stress, eIF4G/eIF4A and PABP remain associated to the eIF4E, but the association with other 43S pre-initiation factors decreases, indicating that ribosome attachment is impaired. Most eIF4E3-associated mRNAs encode for metabolic proteins, while eIF4E4 associate to mRNAs encoding ribosomal proteins. Interestingly, for both eIF4E3/4, more mRNAs were associated in stressed cells than in non-stressed cells, even though these mRNAs have lower translational efficiencies in stress. In summary, trypanosomes have two co-existing eIF4F complexes involved in translation of distinct mRNA cohorts important for growth. Under stress conditions, both complexes exit translation but remain bound to their mRNA targets.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_14:10:29.321.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bernardo Papini Gabiatti
SpeciesList	scientific name: Trypanosoma cruzi Dm28c; NCBI TaxID: 1416333;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-15 03:19:01	ID requested
⏵ 1	2025-05-06 14:10:30	announced

Gabiatti BP, Freire ER, Ferreira da Costa J, Ferrarini MG, Reichert Assun, ç, ã, o de Matos T, Preti H, Munhoz da Rocha I, Guimar, ã, es BG, Kramer S, Zanchin NIT, Holetz FB, Trypanosoma cruzi eIF4E3- and eIF4E4-containing complexes bind different mRNAs and may sequester inactive mRNAs during nutritional stress. Nucleic Acids Res, 53(2):(2025) [pubmed]

10.1093/nar/gkae1181;

submitter keyword: nutritional stress,Trypanosoma, eIF4F, translation initiation

Gene Expression Regulation Laboratory
contact affiliation	Carlos Chagas Institute (ICC), FIOCRUZ/PR, Curitiba, Brazil
contact email	fabiola.holetz@fiocruz.br
lab head
Bernardo Papini Gabiatti
contact affiliation	University of Würzburg
contact email	bernardo.gabiatti@uni-wuerzburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051440
     1. Label: PRIDE project
     2. Name: Two distinct Trypanosoma eIF4F complexes co-exist, bind different mRNAs and are regulated during nutritional stress