PXD051436

PXD051436 is an original dataset announced via ProteomeXchange.

Title	Quantitative Site-specific N-glycosylation Analysis Reveals IgG Glyco-signatures for Pancreatic Cancer Diagnosis
Description	Pancreatic cancer is a highly aggressive tumour with a poor prognosis due to low early detection rates and a lack of biomarkers. Alterations in the N-glycosylation of plasma immunoglobulin G (IgG) have been shown to be closely associated with the onset and development of several cancers and could be used as biomarkers for early diagnosis. In this work, we used a well-evaluated approach (termed GlycoQuant) for the site-specific N-glycosylation profile of human plasma IgG in both healthy individuals and patients with pancreatic ductal adenocarcinoma (PDAC). By analyzing the rapidly purified IgG samples from 100 patients with different stages of PDAC, as well as 30 healthy controls, it was found that the combination of carbohydrate antigen 19-9 (CA19-9), IgG1-GP05 (IgG1-TKPREEQYNSTYR-HexNAc(4)Hex(5)Fuc(1)NeuAc(1)) and IgG3-GP03 (IgG3-EEQYNSTFR-HexNAc(4)Hex(5)Fuc(1)) can be used to distinguish between PDAC patients and healthy individuals (ROC=0.989). Additionally, the combination of CA19-9 and IgG1-GP05 can be used to differentitate between PDAC stage Ⅰ patients and healthy individuals (ROC=0.967). The study showed that the integrated quantitative method can be used for large-scale clinical N-glycoproteomic research to discover new N-glycosylated biomarkers. This could advance the development of clinical glycoproteomics.
HostingRepository	iProX
AnnounceDate	2024-04-15
AnnouncementXML	Submission_2024-04-14_23:46:58.608.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yong Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	deaminated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-14 23:46:19	ID requested
⏵ 1	2024-04-14 23:46:59	announced

Dataset with its publication pending

submitter keyword: Immunoglobulin G, Pancreatic ductal adenocarcinoma, N-glycosylation, Mass spectrometry

Yong Zhang
contact affiliation	West China Hospital, Sichuan University
contact email	nankai1989@foxmail.com
lab head
Yong Zhang
contact affiliation	West China Hospital, Sichuan University
contact email	nankai1989@foxmail.com
dataset submitter

iProX dataset URI