PXD051416 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Engineering of TurboID-Wingless for the identification of Wingless interactors through in vivo proximity labelling |
| Description | Wnt signalling coordinates growth and cell fate decisions during development and mis-regulation of Wnt signalling in adults is associated with a range of conditions, including cancer and neurogenerative diseases. Therefore, means of modulating Wnt proteins and/or cofactors could have significant therapeutic potential. As a first step towards enumerating the Wnt interactome, we devised an in vivo proximity labelling strategy to identify proteins that interact with Wingless (Wg), the main Drosophila Wnt. We engineered the wingless locus to express a functional TurboID-Wg fusion at endogenous levels and identified in vivo interactors by streptavidin pull-down from embryos, followed by mass spectrometry. Further analysis may in future extend the screen coverage and deliver functional validation of the newly identified interactors. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-20 |
| AnnouncementXML | Submission_2026-08-19_22:38:28.600.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mark Skehel |
| SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: NEWT:7227; |
| ModificationList | biotinylated residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-04-12 19:08:32 | ID requested | |
| ⏵ 1 | 2026-08-19 22:38:29 | announced | |
Publication List
Keyword List
| submitter keyword: Wnt, proximity labelling, turboID |
Contact List
| Jean-Paul Vincent |
|---|
| contact affiliation | The Francis Crick Institute, 1 Midland Road, London, NW1 1AT, U.K. |
| contact email | JP.Vincent@crick.ac.uk |
| lab head | |
| Mark Skehel |
|---|
| contact affiliation | Francis Crick Institute |
| contact email | skehelm@crick.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD051416
- Label: PRIDE project
- Name: Engineering of TurboID-Wingless for the identification of Wingless interactors through in vivo proximity labelling
