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PXD051379

PXD051379 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDysregulation of N-glycosylation by RPN1 knockout in spermatocytes induces male infertility via impaired mitochondrial function in mice
DescriptionN-glycosylation protein modifications play a crucial regulatory role in numerous biological processes, although their contribution to male reproduction in mammals remains largely undefined. Here, we found that Ribophorin I (RPN1), a subunit of oligosaccharyltransferase complex, is indispensable for spermatogenesis in male germ cells. Germ cell-specific Rpn1 knockout results in significant inhibition of the initiation and progression of meiosis, consequently disrupting homologous chromosome pairing, meiotic recombination, and DNA double-strand break repair during meiosis. N-glycoproteomic profiling revealed that glycosylation levels are reduced in mitochondria-associated proteins, while functional analyses showed that Rpn1 deficiency could inhibit mitochondrial function and abundance, decreasing spermatocyte populations, and potentially reducing energy availability during meiosis and increasing apoptosis levels in mice. These findings highlight the essential physiological functions of N-glycosylation modification in male spermatogenesis and expand our understanding of its role in male fertility.
HostingRepositoryPRIDE
AnnounceDate2025-05-06
AnnouncementXMLSubmission_2025-05-06_13:34:09.340.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterShan Wang
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListcomplex glycosylation
InstrumenttimsTOF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-11 18:50:57ID requested
12025-05-06 13:34:10announced
Publication List
Zhang M, Li M, Li H, Wan Y, Yang S, Ji S, Zhang H, Liu C, Lu G, Jiang X, Liu H, knockout in spermatocytes induces male infertility via endoplasmic reticulum stress in mice. Int J Biol Sci, 21(5):2360-2379(2025) [pubmed]
10.7150/ijbs.106468;
Keyword List
submitter keyword: RPN1
N-glycosylation
Meiosis
Spermatogenesis
Fertility
Contact List
Mingyu Zhang
contact affiliationShandong University
contact emailzmyscience@163.com
lab head
Shan Wang
contact affiliationCapital Institute of Pediatrics, Beijing, China
contact emailwsaquarius@163.com
dataset submitter
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