PXD051372

PXD051372 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Delivery of MFF through mitochondria-derived vesicles regulates melanosome maturation
Description	Melanosomes are lysosome-associated organelles that produce and accumulate melanin. Their biogenesis begins with the trafficking of melanogenic proteins from the endo-lysosomal system into non-pigmented vacuoles derived from endosomes. Subsequently, melanosome maturation proceeds with the export and recycling of non-required cargo, through tubular carriers and vesicular fission derived from melanosome membranes. Although constriction events have been observed at melanosome membranes, the mechanisms underlying their fission remain unclear. We found that MFF is delivered to melanosomes by mitochondria-derived vesicles (MDVs) and that it is located at melanosome membrane fission sites. Downregulation of MFF leads to an increase in the size of early melanosomes, accumulation of intracellular melanin, and increased melanosome lumen catabolism. In melanocytes, MFF interactome highlighted its interplay with a complex network of cytoskeletal factors. Inhibition of actin nucleation is indeed sufficient to restore the effects on melanosome size. Our data show for the first time that MDVs-mediated MFF trafficking to melanosomes is necessary for their membrane remodeling and fission, promoting the transition from early to mature stage melanosomes.
HostingRepository	PRIDE
AnnounceDate	2026-05-25
AnnouncementXML	Submission_2026-05-24_17:10:28.968.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francescopaolo Iavarone
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-11 08:30:04	ID requested
⏵ 1	2026-05-24 17:10:30	announced

Publication List

10.1038/s41467-026-70572-3;
Magalh, ã, es Rebelo AP, Maracani A, Greco S, Dal Bello F, Santorelli L, Gerdol M, Pallavicini A, Knedlik T, Schiavon S, Scorrano L, Goff PS, Frezza C, Sviderskaya EV, Grumati P, Giacomello M, MFF budding from mitochondria regulates melanosome size and maturation. Nat Commun, 17(1):(2026) [pubmed]

10.1038/s41467-026-70572-3;

Magalh, ã, es Rebelo AP, Maracani A, Greco S, Dal Bello F, Santorelli L, Gerdol M, Pallavicini A, Knedlik T, Schiavon S, Scorrano L, Goff PS, Frezza C, Sviderskaya EV, Grumati P, Giacomello M, MFF budding from mitochondria regulates melanosome size and maturation. Nat Commun, 17(1):(2026) [pubmed]

Keyword List

submitter keyword: mitochondria,MFF, melanosome

submitter keyword: mitochondria,MFF, melanosome

Contact List

Marta Giacomello
contact affiliation	University of Padova
contact email	marta.giacomello@unipd.it
lab head
Francescopaolo Iavarone
contact affiliation	TIGEM
contact email	f.iavarone@tigem.it
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD051372
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD051372

PRIDE project URI

Repository Record List

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