PXD051362

PXD051362 is an original dataset announced via ProteomeXchange.

Title	FTO inhibition attenuates obstruction-induced kidney fibrosis by downregulating ferroptosis activator ACSL4 and profibrotic factor TGFBI
Description	Analysis by LC-MS/MS shotgun proteomics was performed on lysates from the following samples: 1) Control HK2 cells generated using control CRISPR/CAS9 system and scrambled guide RNA (sgRNAGCACTCACATCGCTACATCA) 2) HK2 cells with FTO knockout using CRISPR/Cas9 system and target 2 guide RNA (sgCCTACAAGTACCTGAACACC) 3) HK2 cells with FTO knockout using CRISPR/Cas9 system and target 3 guide RNA (sgCCAGGCTCTTTACGGTCCCC) 4) HK2 cells treated with vehicle control 5) HK2 cells treated with erastin 6) HK2 cells treated with FTO inhibitor FB23-2
HostingRepository	PRIDE
AnnounceDate	2025-10-13
AnnouncementXML	Submission_2025-10-12_23:46:00.706.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sharon Pitteri
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-11 02:02:57	ID requested
⏵ 1	2025-10-12 23:46:01	announced

10.1016/j.isci.2025.113515;

Zhang D, Chiu CL, Wen R, Qiu Z, Garcia-Marques F, Bermudez A, Zhao M, Zhao H, Dixon SJ, Peehl DM, Rankin EB, Pitteri S, Brooks JD, FTO inhibition attenuates renal fibrosis by downregulating ferroptosis activator ACSL4 and profibrotic factor TGFBI. iScience, 28(10):113515(2025) [pubmed]

submitter keyword: HK2, ferroptosis,kidney fibrosis, FTO

Sharon Pitteri
contact affiliation	Department of Radiology Canary Center at Stanford for Cancer Early Detection Stanford School of Medicine Stanford University
contact email	spitteri@stanford.edu
lab head
Sharon Pitteri
contact affiliation	Stanford University
contact email	spitteri@stanford.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD051362

PRIDE project URI

• PRIDE
  1. PXD051362
     1. Label: PRIDE project
     2. Name: FTO inhibition attenuates obstruction-induced kidney fibrosis by downregulating ferroptosis activator ACSL4 and profibrotic factor TGFBI