PXD051348

PXD051348 is an original dataset announced via ProteomeXchange.

Title	Multi-scale photocatalytic proximity labeling reveals cell surface neighbors on and between cells
Description	Proximity labeling proteomics (PLP) strategies are powerful approaches to yield snapshots of protein neighborhoods. Here, we describe a multi-scale PLP method with adjustable resolution using a commercially available photosensitizer, Eosin Y, which upon visible light illumination, activates different photo-probes with a range of labeling radii. We applied this platform to profile neighborhoods of the oncogenic epidermal growth factor receptor (EGFR) and orthogonally validated >20 neighbors using immuno-assays and AlphaFold-Multimer prediction. We further profiled the protein neighborhoods of cell-cell synapses induced by bi-specific T-cell engagers (BiTEs) and chimeric antigen receptor (CAR)T cells. This integrated multi-scale PLP platform maps local and distal protein networks on and between cell surfaces, which will aid in the systematic construction of the cell surface interactome and reveal new immunotherapeutic opportunities.
HostingRepository	PRIDE
AnnounceDate	2025-02-17
AnnouncementXML	Submission_2025-02-17_04:48:59.720.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhi Lin
SpeciesList	scientific name: Acyrthosiphon pisum (Pea aphid); NCBI TaxID: 7029; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro; timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-10 23:55:40	ID requested
⏵ 1	2025-02-17 04:49:00	announced

10.1126/science.adl5763;

Lin Z, Schaefer K, Lui I, Yao Z, Fossati A, Swaney DL, Palar A, Sali A, Wells JA, Multiscale photocatalytic proximity labeling reveals cell surface neighbors on and between cells. Science, 385(6706):eadl5763(2024) [pubmed]

submitter keyword: Chimeric Antigen Receptor (CAR) T cells, Epidermal Growth Factor Receptor, Proximity labeling,Photocatalysis, Bispecific T cell Engager

James A. Wells
contact affiliation	UCSF
contact email	jim.wells@ucsf.edu
lab head
Zhi Lin
contact affiliation	UCSF
contact email	zhi.lin@ucsf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD051348

PRIDE project URI

• PRIDE
  1. PXD051348
     1. Label: PRIDE project
     2. Name: Multi-scale photocatalytic proximity labeling reveals cell surface neighbors on and between cells